A4355

Monoclonal Anti-ASPP1 antibody produced in mouse

~2 mg/mL, clone LXO54.2, purified immunoglobulin, buffered aqueous solution

• Synonym(s): Anti-Apoptosis-stimulating protein of p53, 1, Anti-PPP1R13B, Anti-protein phosphatase 1 regulatory subunit 13B
• UNSPSC Code: 12352203
• NACRES: NA.41
• MDL number: MFCD08705574
• Conjugate: unconjugated
• Clone: LXO54.2, monoclonal
• Application: ARR, IP, WB
• Citations: 8

Properties

• biological source: mouse
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: LXO54.2, monoclonal
• form: buffered aqueous solution
• mol wt: antigen ~175 kDa
• species reactivity: mouse, human
• concentration: ~2 mg/mL
• technique(s): immunoprecipitation (IP): suitable, microarray: suitable, western blot: 1-2 μg/mL using total cell extract of human osteogenic sarcoma, U-2-OS
• isotype: IgG1
• UniProt accession no.: Q96KQ4
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... PPP1R13B(23368) ; mouse ... Ppp1r13b(21981)

Description

General description

ASPP1 interacts with p53 to enhance p53-induced apoptosis.

Monoclonal Anti-ASPP1 (mouse IgG1 isotype) is derived from the hybridoma LXO54.2 produced by the fusion of mouse myeloma cells (SP2/0 cells) and splenocytes from BALB/c mice immunized with a recombinant fragment of human ASPP1, amino acids. Apoptosis-stimulating protein of p53, 1 (ASPP1) belongs to the ASPP family of proteins. This protein contains a proline-rich region, four ankyrin repeats, and an SH3 domain in the C-terminal end.

Immunogen

recombinant fragment of human ASPP1 (amino acids 1-308).

Application

Monoclonal Anti-ASPP1 antibody can be used for western blot at 1-2 μg/mL using total cell extract of human osteogenic sarcoma, U-2-OS. The antibody can also be used for microarray and immunoprecipitation assays.

Biochem/physiol Actions

Apoptosis-stimulating protein of p53, 1 (ASPP1) is known to stimulate RAS signalling which subsequently enhances p53-dependent apoptosis in cancer cells. Furthermore, ASPP1 can also induce p53-independent apoptosis by enhancing the apoptotic functions of p63 and p73. Monoclonal Anti-ASPP1 antibody recognizes human and mouse ASPP1.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)