Skip to Content
MilliporeSigma
All Photos(1)

Documents

A1227

Sigma-Aldrich

ABTS Enhancer

liquid, for peroxidase detection

Synonym(s):

2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) Enhancer

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

UNSPSC Code:
12352204
NACRES:
NA.83

product name

ABTS Enhancer, for peroxidase detection

Quality Level

100

form

liquid

storage temp.

2-8°C

Application

2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) has been used for the determination of laccase activity in Trametes versicolor. It may be suitable for use in enzyme-linked immunosorbent assay (ELISA) for detecting signal strength emitted by cells.

Biochem/physiol Actions

2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) is a non-phenolic substrate. It might exhibit antioxidant property. The detection of the horseradish peroxidase substrate ABTS can be improved by the addition of a chemical enhancer. The ABTS enhancer increases the absorbance of the oxidized product 50-150%. This increase is used to improve assay sensitivity or conserve assay components.

Physical form

Supplied as an 11X concentrate

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H350,H412

Hazard Classifications

Aquatic Chronic 3 - Carc. 1B

Storage Class

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Mark E Levenstein et al.
Stem cells (Dayton, Ohio), 26(12), 3099-3107 (2008-09-20)
Human embryonic stem (ES) cells can be maintained in an undifferentiated state if the culture medium is first conditioned on a layer of mouse embryonic fibroblast (MEF) feeder cells. Here we show that human ES cell proliferation is coordinated by
Investigations on antioxidant potential of phenolic acids and flavonoids: the common phytochemical ingredients in plants
Singh DP, et al.
Journal of Plant Biochemistry & Physiology (2018)
Khaled K Abu-Amero et al.
Molecular vision, 17, 2769-2775 (2011-11-09)
To evaluate total antioxidant status (TAS) in the plasma of pseudoexfoliation glaucoma (PEG) patients and to compare this level with a matching control group. Additionally, we aim to investigate the effect of the combined action of the lysyl oxidase-like 1
Manickam Naresh Kumar et al.
Bioresource technology, 244(Pt 1), 353-361 (2017-08-07)
Inhibitor mediated intensified bio-pretreatment (IMBP) technology using natural cellulase inhibitor (NCI) for maximum cellulose recovery from paddy straw was studied. Pretreatment was carried out under solid state condition. Supplementation of 8% NCI in pretreatment medium improves cellulose recovery and delignification
Meng-Hsuan Wu et al.
Scientific reports, 9(1), 9754-9754 (2019-07-07)
Laccases that are tolerant to organic solvents are powerful bio-catalysts with broad applications in biotechnology. Most of these uses must be accomplished at high concentration of organic solvents, during which proteins undergo unfolding, thereby losing enzyme activity. Here we show

Articles

Colorimetric Phosphatase/Peroxidase Substrate Systems

NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service