91077C
Insulin, Human Recombinant
for research or for further manufacturing use, dry powder
Synonym(s):
Insulin sodium salt human, rh-Insulin
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100 MG
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$373.00
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$1,040.00
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$3,420.00
About This Item
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InChI key
PBGKTOXHQIOBKM-FHFVDXKLSA-N
form
dry powder
potency
≥27.5 units per mg
Quality Level
Gene Information
human ... INS(3630)
quality
300
technique(s)
cell culture | mammalian: suitable
impurities
≤1.0 EU/mL endotoxin
solubility
water: 10 mg/mL (pH 2.0—2.5)
UniProt accession no.
shipped in
ambient
storage temp.
−20°C
1 of 4
This Item | |||
|---|---|---|---|
| form dry powder | form powder | form solution | form - |
| impurities ≤1.0 EU/mL endotoxin | impurities - | impurities ≤50 EU/mL endotoxin | impurities - |
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) - |
| potency ≥27.5 units per mg | potency - | potency - | potency - |
| solubility water: 10 mg/mL (pH 2.0—2.5) | solubility 0.01 M HCl: 0.5 mg/mL, clear to slightly hazy, colorless | solubility - | solubility - |
recombinant human insulin, serum free media supplement, SFM supplement | - | recombinant human insulin | - |
General description
Insulin, recombinant human (rHu) is identical in function and structure to the native human sequence. A hormone consisting of two polypeptide chains, rHu Insulin′s A-chain (21 amino acids) and B-chain (30 amino acids) are covalently linked by disulfide bonds between cysteine residues.
Application
Serum-free medium supplements such as insulin are essential for long-term growth of commonly used mammalian cell lines. When insulin is absent from media, cell may exhibit disturbances in morphology and growth rate.
- Recombinant Human Insulin has been used in the media for adipogenic induction of mesenchymal stem cells.[1]
- It has been used as a supplement in the cell culture media of airway epithelial cells.
- It has been used in the culture media for differentiation of cells, GLUT4 (glucose transporter type 4) translocation assay and to study interaction of insulin with insulin receptor via surface plasmon resonance.[2]
Biochem/physiol Actions
Two-chain polypeptide hormone produced by the β-cells of pancreatic islets. Its molecular weight is ~5800 Da. The α and β chains are joined by two interchain disulfide bonds. The α chain contains an intrachain disulfide bond. Insulin regulates the cellular uptake, utilization, and storage of glucose, amino acids, and fatty acids and inhibits the breakdown of glycogen, protein, and fat.
Other Notes
InChI: 1S/C257H383N65O77S6/c1-29-131(23)205(313-193(339)104-259)252(393)317-204(130(21)22)248(389)288-159(75-82-200(349)350)217(358)282-156(71-78-189(263)335)221(362)308-183-116-403-404-117-184-243(384)305-178(111-324)240(381)294-162(88-123(7)8)225(366)295-168(95-140-53-61-146(329)62-54-140)228(369)283-154(69-76-187(261)333)218(359)290-161(87-122(5)6)223(364)285-158(74-81-199(347)348)220(361)302-174(101-190(264)336)235(376)298-170(97-142-57-65-148(331)66-58-142)231(372)309-182(242(383)304-176(255(396)397)103-192(266)338)115-402-400-113-180(307-226(367)163(89-124(9)10)291-232(373)172(99-144-105-269-119-275-144)300-219(360)155(70-77-188(262)334)284-234(375)175(102-191(265)337)303-249(390)201(127(15)16)314-211(352)150(260)92-137-44-34-31-35-45-137)213(354)274-109-196(342)280-177(110-323)239(380)301-173(100-145-106-270-120-276-145)233(374)293-165(91-126(13)14)237(378)315-202(128(17)18)247(388)287-157(73-80-198(345)346)215(356)277-133(25)210(351)289-160(86-121(3)4)224(365)296-169(96-141-55-63-147(330)64-56-141)229(370)292-164(90-125(11)12)236(377)316-203(129(19)20)250(391)311-181(114-401-405-118-185(310-244(183)385)245(386)319-207(134(26)326)253(394)306-179(112-325)241(382)318-206(132(24)30-2)251(392)312-184)214(355)273-107-194(340)278-153(72-79-197(343)344)216(357)281-151(51-42-84-271-257(267)268)212(353)272-108-195(341)279-166(93-138-46-36-32-37-47-138)227(368)297-167(94-139-48-38-33-39-49-139)230(371)299-171(98-143-59-67-149(332)68-60-143)238(379)320-208(135(27)327)254(395)322-85-43-52-186(322)246(387)286-152(50-40-41-83-258)222(363)321-209(136(28)328)256(398)399/h31-39,44-49,53-68,105-106,119-136,150-186,201-209,323-332H,29-30,40-43,50-52,69-104,107-118,258-260H2,1-28H3,(H2,261,333)(H2,262,334)(H2,263,335)(H2,264,336)(H2,265,337)(H2,266,338)(H,269,275)(H,270,276)(H,272,353)(H,273,355)(H,274,354)(H,277,356)(H,278,340)(H,279,341)(H,280,342)(H,281,357)(H,282,358)(H,283,369)(H,284,375)(H,285,364)(H,286,387)(H,287,388)(H,288,389)(H,289,351)(H,290,359)(H,291,373)(H,292,370)(H,293,374)(H,294,381)(H,295,366)(H,296,365)(H,297,368)(H,298,376)(H,299,371)(H,300,360)(H,301,380)(H,302,361)(H,303,390)(H,304,383)(H,305,384)(H,306,394)(H,307,367)(H,308,362)(H,309,372)(H,310,385)(H,311,391)(H,312,392)(H,313,339)(H,314,352)(H,315,378)(H,316,377)(H,317,393)(H,318,382)(H,319,386)(H,320,379)(H,321,363)(H,343,344)(H,345,346)(H,347,348)(H,349,350)(H,396,397)(H,398,399)(H4,267,268,271)
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Storage Class
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wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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