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78102

Sigma-Aldrich

Anti-Mouse IgG - Atto 633 antibody produced in goat

~1 mg/mL IgG, affinity isolated antibody

Synonym(s):

Anti-Mouse IgG−Atto 633 antibody produced in goat, Atto 633 [goat-Anti-mouse IgG]

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.46

biological source

goat

conjugate

Atto 633 conjugate

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

contains

50% glycerol as stabilizer

species reactivity

mouse

concentration

~1 mg/mL IgG

fluorescence

λex 636 nm; λem 650 nm in PBS

storage temp.

−20°C

target post-translational modification

unmodified

Related Categories

General description

Immunoglobulin G (IgG) is a glycoprotein antibody that regulates immune responses such as phagocytosis and is also involved in the development of autoimmune diseases. Mouse IgGs have four distinct isotypes, namely, IgG1, IgG2a, IgG2b, and IgG3. IgG1 regulates complement fixation in mice.
Anti-mouse IgG (whole molecule) is purified from goat anti-mouse IgG antiserum to remove essentially all goat serum proteins, including immunoglobulins, which do not specifically bind to mouse IgG. The antibody preparation is solid phase adsorbed with human serum proteins to ensure minimal cross reactivity. Goat anti-mouse is conjugated to Atto 633 NHS and purified by gel permeation chromatography and dialysis to remove unbound dye. Atto 633-goat anti mouse IgG associates with mouse IgGs.

Immunogen

purified mouse IgG (whole molecule).

Application

Atto fluorescent labels are designed for high sensitivity applications, including single molecule detection. Atto labels have rigid structures that do not show any cis-trans-isomerization. Thus these labels display exceptional intensity with minimal spect

Physical form

Solution in phosphate buffered saline and 5 mM sodium azide, pH 7.5, with glycerol 1:1

Analysis Note

Unconjugated dye ≤5% of total fluorescence

Legal Information

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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hcodes

H412

Hazard Classifications

Aquatic Chronic 3

Storage Class

10 - Combustible liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Charlotte Calvet et al.
iScience, 25(12), 105628-105628 (2022-12-10)
Hearing depends on fast and sustained calcium-dependent synaptic vesicle fusion at the ribbon synapses of cochlear inner hair cells (IHCs). The implication of the canonical neuronal SNARE complex in this exocytotic process has so far remained controversial. We investigated the
Tharun Selvam Mahendran et al.
Frontiers in aging neuroscience, 12, 537712-537712 (2020-12-01)
Aggregated tau is a hallmark neuropathological feature in numerous neurodegenerative disorders. Previous studies aiming to validate aggregated tau pathology as a pathogenic driver of neurodegeneration in correlation to characteristic behavioral phenotypes have had shortcomings. Although studies on soluble tau pathology
Complement-fixing IgG1 constitutes a new subclass of mouse IgG.
P L Ey et al.
Nature, 281(5731), 492-493 (1979-10-11)
Structure and function of human and murine receptors for IgG.
J C Unkeless et al.
Annual review of immunology, 6, 251-281 (1988-01-01)

Articles

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