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69125

Sigma-Aldrich

Resorufin methyl ether

suitable for fluorescence, ≥98.0% (HPLC)

Synonym(s):

7-Methoxy-3H-phenoxazin-3-one, Methoxyresorufin, O7-Methylresorufin

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About This Item

Empirical Formula (Hill Notation):
C13H9NO3
CAS Number:
5725-89-3
Molecular Weight:
227.22
Beilstein/REAXYS Number:
209529
MDL number:
MFCD00037663
UNSPSC Code:
12352200
PubChem Substance ID:
57652175

assay

≥98.0% (HPLC)

form

crystals

mp

≥220 °C (lit.)

solubility

DMF: soluble
DMSO: soluble
alcohols: soluble

fluorescence

λex 571 nm; λem 585 nm in dealkylase(lit.)

suitability

suitable for fluorescence

storage temp.

2-8°C

SMILES string

COc1ccc2N=C3C=CC(=O)C=C3Oc2c1

InChI

1S/C13H9NO3/c1-16-9-3-5-11-13(7-9)17-12-6-8(15)2-4-10(12)14-11/h2-7H,1H3

InChI key

KNYYMGDYROYBRE-UHFFFAOYSA-N

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Application

Used to differentiate isozymes of cytochrome P-450

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Storage Class

13 - Non Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
BCBW5768
BCBT7951
BCBP7333V
BCBN7818V
BCBM0670V

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A G Krainev et al.
Archives of biochemistry and biophysics, 298(1), 198-203 (1992-10-01)
Some eukaryotic cytochromes P450 (P450s) have a series of ionic amino acids, corresponding to Lys250, Arg251, and Lys253 residues in the P450 1A2 sequence. To understand the roles of those ionic amino acids in the catalytic function of P450, three
D F Staskal et al.
Toxicological sciences : an official journal of the Society of Toxicology, 84(2), 225-231 (2005-01-22)
Dioxins have been shown to bind and induce rodent CYP1A2, producing a dose-dependent hepatic sequestration in vivo. The induction of CYP1A2 activity has been used as a noninvasive biomarker for human exposure to dioxins; while there is a consistent relationship
A Toby A Jenkins et al.
Bioelectrochemistry (Amsterdam, Netherlands), 68(1), 67-71 (2005-06-07)
This paper describes the utilisation of methoxy-resorufin ether as an electrochemical probe for studying cytochrome P450 CYP6G1. Methoxy-resorufin ether is well established as a versatile substrate for cytochrome P450, as its demethylated product, resorufin, is a fluorophore. We show that
Xiaowei Zhang et al.
Toxicology and applied pharmacology, 234(3), 306-313 (2008-12-02)
As part of an ongoing effort to understand aryl hydrocarbon receptor (AhR) mediated toxicity in mink, cDNAs encoding for CYP1A1 and the CYP1A2 mixed function monooxygenases were cloned and characterized. In addition, the effects of selected dibenzofurans on the expression
M Teresa Donato et al.
Drug metabolism and disposition: the biological fate of chemicals, 34(9), 1556-1562 (2006-06-10)
Liver grafts discarded for transplantation because of macrosteatosis can constitute a valuable source of human hepatocytes for in vitro metabolic and pharmacotoxicological studies or for therapeutic applications. A condition for using hepatocyte suspensions for these purposes is the preservation of
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