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Dulbecco′s Modified Eagle′s Medium/High Modified

with 4500 mg/L dextrose, with 4.0 mM L-glutamine, with 110 mg/L sodium pyruvate, without sodium bicarbonate, powder, suitable for cell culture

Pharma Manufacturing

Synonym(s):

Dulbecco’s Modified Eagle’s Medium - high glucose, DME, DMEM

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About This Item

UNSPSC Code:
12352200
NACRES:
NA.71

description

for research or for further manufacturing use

Quality Level

500

form

powder

technique(s)

cell culture | mammalian: suitable

components

phenol red: yes
sodium pyruvate: yes
L-glutamine: yes
glucose: high

shipped in

ambient

storage temp.

2-8°C

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Application

Dulbecco′s Modified Eagle′s Medium (DMEM) is a modification of Basal Medium Eagle (BME) that contains four-fold concentrations of the amino acids and vitamins. The original formulation contained 1000 mg/L of glucose and was used to culture embryonic mouse cells. Since then, it has been modified in several ways to support primary cultures of mouse and chicken cells, as well as a variety of normal and transformed cells. Each of these media offers a different combination of L-glutamine and sodium pyruvate. Additionally, the glucose levels have been raised to 4500 mg/L, contributing to the name "DMEM/High".

Quantity

Formulated to contain 13.5 grams of powder per liter of medium.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

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Mengxi Jiang et al.
Proceedings of the National Academy of Sciences of the United States of America, 117(26), 15055-15065 (2020-06-20)
Phosphocholine phosphatase-1 (PHOSPHO1) is a phosphocholine phosphatase that catalyzes the hydrolysis of phosphocholine (PC) to choline. Here we demonstrate that the PHOSPHO1 transcript is highly enriched in mature brown adipose tissue (BAT) and is further induced by cold and isoproterenol
Marko Knoll et al.
Nature communications, 8(1), 2115-2115 (2017-12-14)
Brown adipose tissue (BAT) metabolism influences glucose homeostasis and metabolic health in mice and humans. Sympathetic stimulation of β-adrenergic receptors in response to cold induces proliferation, differentiation, and UCP1 expression in pre-adipocytes and mature brown adipocytes. Here we show that
Maria Masnata et al.
Acta neuropathologica, 137(6), 981-1001 (2019-02-23)
In recent years, evidence has accumulated to suggest that mutant huntingtin protein (mHTT) can spread into healthy tissue in a prion-like fashion. This theory, however, remains controversial. To fully address this concept and to understand the possible consequences of mHTT spreading

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