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40809

Sigma-Aldrich

Purine nucleoside phosphorylase

recombinant, expressed in E. coli, ≥120 U/mL

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About This Item

CAS Number:
9030-21-1
Enzyme Commission number:
2.4.2.1 (BRENDA, IUBMB)
MDL number:
MFCD00131739
UNSPSC Code:
12352204

recombinant

expressed in E. coli

form

buffered aqueous solution (100 mM phosphate buffer with 1 mM MgCl2)

specific activity

≥120 U/mL

storage temp.

−20°C

InChI

1S/C10H12N4O4/c15-2-6-7(16)8(17)10(18-6)14-4-13-5-1-11-3-12-9(5)14/h1,3-4,6-8,10,15-17H,2H2/t6-,7-,8-,10-/m1/s1

InChI key

MRWXACSTFXYYMV-FDDDBJFASA-N

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Application

Purine nucleoside phosphorylase is used to study nucleotide salvage pathways and purine metabolism[1]. It is used to study purine nucleoside phosphorylase deficiency and responsible PNP genetic mutations[2].

Biochem/physiol Actions

Purine nucleoside phosphorylase is an enzyme involved in purine metabolism. PNP metabolizes adenosine into adenine, inosine into hypoxanthine, and guanosine into guanine[1]. Mutations in the PNP gene are responsible for purine nucleoside phosphorylase deficiency[2].

Unit Definition

1 U will cause the phosphorolysis 1 μmol of inosine to hypoxanthine and ribose 1-phosphate per minute at pH 7.4 at 25°C

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
BCBQ2936V
BCBN7658V
BCBL2387V
BCBJ7734V
BCBD8998

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Purine nucleoside phosphorylase deficiency with a novel PNP gene mutation: a first case report from India
Manisha Rajan Madkaikar, et al.
BMJ Case Reports, DOI:10-DOI:10 (2011)
Fernanda Canduri et al.
Biochemical and biophysical research communications, 313(4), 907-914 (2004-01-07)
Human purine nucleoside phosphorylase (PNP) is a ubiquitous enzyme which plays a key role in the purine salvage pathway, and PNP deficiency in humans leads to an impairment of T-cell function, usually with no apparent effect on B-cell function. PNP
A M Ledgard et al.
Reproduction, fertility, and development, 24(7), 962-972 (2012-09-01)
The uterus provides the nurturing environment that supports the growth of the early preimplantation bovine conceptus. To determine critical time points of uterine influence, in vitro-produced Day 7 blastocysts were transferred into synchronous (Day 7) uteri and asynchronous uteri (Days
Candida Deves et al.
Bone, 52(1), 167-175 (2012-10-03)
Purine nucleoside phosphorylase (PNP) is a purine-metabolizing enzyme that catalyzes the reversible phosphorolysis of 6-oxypurine (deoxy)nucleosides to their respective bases and (deoxy)ribose-1-phosphate. It is a key enzyme in the purine salvage pathway of mammalian cells. The present investigation sought to
K K Jason Chan et al.
Methods in enzymology, 516, 219-235 (2012-10-05)
Nature has hardly evolved a biochemistry of fluorine although there is a low-level occurrence of fluoroacetate found in selected tropical and subtropical plants. This compound, which is generally produced in low concentrations, has been identified in the plants due to
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  1. What is the source gene of the purine nucleoside phosphorylase, Product 40809?

    1 answer

    1. Per our supplier, the purine nucleoside phosphorylase,product 40809, is of bacterial origin, specifically from Enterobacter.

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  2. What is the Department of Transportation shipping information for this product?

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    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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