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21966

Sigma-Aldrich

5(6)-Carboxy-X-rhodamine N-succinimidyl ester

BioReagent, suitable for fluorescence

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About This Item

Empirical Formula (Hill Notation):
C37H33N3O7
CAS Number:
114616-32-9
Molecular Weight:
631.67
UNSPSC Code:
12352200
PubChem Substance ID:
329752229

product line

BioReagent

solubility

DMF: soluble
acetonitrile: soluble

fluorescence

λex 575 nm; λem 605 nm in 0.1 M phosphate pH 7.0

suitability

suitable for fluorescence

storage temp.

−20°C

SMILES string

O=C1CCC(=O)N1OC(=O)c2ccc3c(c2)C(=O)OC34c5cc6CCCN7CCCc(c5Oc8c9CCCN%10CCCc(cc48)c9%10)c67.O=C%11CCC(=O)N%11OC(=O)c%12ccc%13C(=O)OC%14(c%15cc%16CCCN%17CCCc(c%15Oc%18c%19CCCN%20CCCc(cc%14%18)c%19%20)c%16%17)c%13c%12

InChI

1S/2C37H33N3O7/c41-29-11-12-30(42)40(29)47-35(43)22-9-10-26-25(17-22)36(44)46-37(26)27-18-20-5-1-13-38-15-3-7-23(31(20)38)33(27)45-34-24-8-4-16-39-14-2-6-21(32(24)39)19-28(34)37;41-29-11-12-30(42)40(29)47-35(43)22-9-10-23-26(19-22)37(46-36(23)44)27-17-20-5-1-13-38-15-3-7-24(31(20)38)33(27)45-34-25-8-4-16-39-14-2-6-21(32(25)39)18-28(34)37/h2*9-10,17-19H,1-8,11-16H2

InChI key

YEWCZAUCUAYJIO-UHFFFAOYSA-N

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Application

5(6)-Carboxy-X-rhodamine N-succinimidyl ester may used as a labeling reagent to attach the fluorophore 5(6)-carboxy-X-rhodamine to target molecules such as nucleic acids. 5(6)-Carboxy-X-rhodamine labeled molecules may be used in fluorescence energy transfer (FRET) applications.

pictograms

Exclamation mark
GHS07

signalword

Warning

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

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S C Hung et al.
Analytical biochemistry, 255(1), 32-38 (1998-02-04)
Fluorescence energy transfer (ET) primers are far superior to single dye-labeled primers as labels for DNA sequencing and polymerase chain reaction amplification. We compare here ET primers with different donor and acceptor dye combinations with respect to the relative acceptor
S C Hung et al.
Analytical biochemistry, 252(1), 78-88 (1997-11-05)
We have synthesized and characterized the spectroscopic properties of 56 energy transfer (ET) fluorescent dye-labeled primers differing in (i) the spacing between the donor and acceptor, (ii) the nature of the spacer (either oligonucleotide or polydideoxyribose phosphate), (iii) the primer
Tae Seok Seo et al.
Proceedings of the National Academy of Sciences of the United States of America, 102(17), 5926-5931 (2005-04-15)
We report four-color DNA sequencing by synthesis (SBS) on a chip, using four photocleavable fluorescent nucleotide analogues (dGTP-PC-Bodipy-FL-510, dUTP-PC-R6G, dATP-PC-ROX, and dCTP-PC-Bodipy-650) (PC, photocleavable; Bodipy, 4,4-difluoro-4-bora-3alpha,4alpha-diaza-s-indacene; ROX, 6-carboxy-X-rhodamine; R6G, 6-carboxyrhodamine-6G). Each nucleotide analogue consists of a different fluorophore attached to
Hailong Li et al.
Biosensors & bioelectronics, 27(1), 167-171 (2011-07-26)
In this article, we report on the first use of fluorescence resonance energy transfer (FRET) dye-labeled probe for fluorescence resonance enhanced DNA detection to greatly improve discrimination ability toward single-base mismatch using conjugation polymer poly(p-phenylenediamine) nanobelts (PNs) as a sensing

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