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17751

Sigma-Aldrich

3-(1-Acetylacetonylazo)phthalhydrazide

BioReagent, suitable for fluorescence, ≥97.0% (HPLC)

Synonym(s):

3-(1,4-Dioxo-1,2,3,4-tetrahydrophthalazin-5-ylazo)-2,4-pentanedione

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About This Item

Empirical Formula (Hill Notation):
C13H12N4O4
Molecular Weight:
288.26
MDL number:
UNSPSC Code:
12352200
PubChem Substance ID:

description

luminescence test: Emmax475 nm 0.1M Tris pH 8.5; after addition of H2O2

product line

BioReagent

assay

≥97.0% (HPLC)

mp

>300 °C

suitability

suitable for fluorescence

SMILES string

CC(=O)C(\N=N\c1cccc2C(=O)NNC(=O)c12)C(C)=O

InChI

1S/C13H12N4O4/c1-6(18)11(7(2)19)15-14-9-5-3-4-8-10(9)13(21)17-16-12(8)20/h3-5,11H,1-2H3,(H,16,20)(H,17,21)/b15-14+

InChI key

XWZORHXCJSIQCY-CCEZHUSRSA-N

Other Notes

Chemiluminescent diagnostic reagent for assaying Helicobacter pylori, which produces urease; much more sensitive than other methods

pictograms

Skull and crossbones

signalword

Danger

hcodes

Hazard Classifications

Acute Tox. 3 Oral

Storage Class

6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Faceshields, Gloves, type P2 (EN 143) respirator cartridges


Certificates of Analysis (COA)

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A Roda et al.
Analytical biochemistry, 264(1), 47-52 (1998-10-24)
A chemiluminescent urease activity assay has been developed and optimized using the chemiluminescent pH indicator phthalhydrazidylazoacetylacetone. This compound is stable at pH </= 7 and decomposes at higher pH values, emitting light in the presence of H2O2. Urease catalyzes hydrolysis

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