This product is soluble in water. No additional calcium is required. For specific applications it may also be prepared in typical sodium or potassium phosphate buffer solutions.
10069
α-Amylase from Bacillus sp.
powder, yellow-brown, ~380 U/mg
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250 MG
$126.00
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$362.18
$126.00
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250 MG
$126.00
1 G
$362.18
About This Item
$126.00
In StockDetails
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biological source
Bacillus sp.
Quality Level
form
powder
specific activity
~380 U/mg
greener alternative product characteristics
Waste Prevention
Design for Energy Efficiency
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sustainability
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color
yellow-brown
greener alternative category
, Enabling
storage temp.
2-8°C
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General description
α-Amylase (α-1,4-glucan-4-glucanohydrolase) belongs to the glycosyl hydrolase family 13. The two aspartic residues and one glutamic acid residue are the prime catalytic residues of α-amylase.[1] All amylases have three domain regions, namely, domain A with a central (β/α)8 barrel, domain B, and β-structure with a Greek key motif encompassing domain C.[1]
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been enhanced for energy efficiency and waste prevention when used in starch hydrolysis research. For more information see the article in biofiles.
Application
Biochem/physiol Actions
α-Amylase mediates the hydrolysis of starch, malto-oligosaccharides, and glycogen at the α-D-(1,4)-glucosidic linkages.[1] Bacillus sp. serve as an important cell factory for the heterogeneous production of α-amylase.[5] An extracellular secreted thermostable amylase from the Bacillus subtilis strain has also been reported.[6]
Unit Definition
One unit is the amount of enzyme which liberates 1 μmole of maltose per minute at pH 6.9 and 25°C (using Cat. No. 85642 as substrate)
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Storage Class
11 - Combustible Solids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
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Masayuki Kagawa et al.
Journal of bacteriology, 185(23), 6981-6984 (2003-11-18)
The crystal structure of Bacillus subtilis alpha-amylase, in complex with the pseudotetrasaccharide inhibitor acarbose, revealed an hexasaccharide in the active site as a result of transglycosylation. After comparison with the known structure of the catalytic-site mutant complexed with the native
Shaomin Yan et al.
Microbial cell factories, 16(1), 124-124 (2017-07-21)
Amylase plays an important role in biotechnology industries, and Gram-positive bacterium Bacillus subtilis is a major host to produce heterogeneous α-amylases. However, the secretion stress limits the high yield of α-amylase in B. subtilis although huge efforts have been made
P.L. Chang Rupp et al.
Journal of Food Biochemistry, 12, 191-191 (1988)
Cold-water solubility, oil-adsorption and enzymolysis properties of amorphous granular starches
Fang C, et al.
Food Hydrocolloids, 117, 106669-106669 (2021)
Junrong Huang et al.
Carbohydrate polymers, 163, 324-329 (2017-03-08)
After combined hydrolysis by α-amylase and β-amylase at room temperature, spherical blocklets in diameters of 27-60nm were observed on the surface of tapioca starch granules by scanning electron micrography (SEM). Tapioca starch (1%, w/w, db, distilled water) was heated by
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In which solution I should to dissolve the alfa amylase? should had ca+2? (for starch degradation)
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