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04272

Sigma-Aldrich

di-Sodium hydrogen phosphate dihydrate

meets analytical specification of Ph. Eur., BP, 98.5-101% (calc. to the dried substance)

Synonym(s):

sec-Sodium phosphate, Disodium hydrogen phosphate dihydrate, Disodium phosphate, Sodium phosphate dibasic dihydrate

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About This Item

Linear Formula:
Na2HPO4 · 2H2O
CAS Number:
10028-24-7
Molecular Weight:
177.99
EC Number:
231-448-7
MDL number:
MFCD00149182
UNSPSC Code:
12161700
PubChem Substance ID:
329747906
NACRES:
NA.25

agency

meets analytical specifications of BP
meets analytical specifications of Ph. Eur.

assay

98.5-101% (calc. to the dried substance)

form

crystals

quality

meets analytical specification of Ph. Eur., BP

impurities

reducing matter, complies
 residual solvents, complies
≤0.001% heavy metals (as Pb)
≤1% NaH2PO4 (calc. to dried subst.)

loss

19.5-21% loss on drying, 130 °C

pH

9.0-9.4 (20 °C, 1%)

anion traces

chloride (Cl-): ≤50 mg/kg
sulfate (SO42-): ≤100 mg/kg

cation traces

As: ≤2 mg/kg
Fe: ≤10 mg/kg

suitability

complies for appearance of solution

SMILES string

O.O.[Na+].[Na+].OP([O-])([O-])=O

InChI

1S/2Na.H3O4P.2H2O/c;;1-5(2,3)4;;/h;;(H3,1,2,3,4);2*1H2/q2*+1;;;/p-2

InChI key

KDQPSPMLNJTZAL-UHFFFAOYSA-L

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Application

Sodium phosphate dibasic dihydrate has been used for isolating DNA and RNA from bacterial samples of human gastrointestinal (GI) tract.

Biochem/physiol Actions

Sodium phosphate dibasic dihydrate is an important component of running buffer of denaturing gel electrophoresis.

Storage Class

13 - Non Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

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Erwin G Zoetendal et al.
Nature protocols, 1(2), 954-959 (2007-04-05)
The human gastrointestinal (GI) tract contains a complex microbial community that consists of numerous uncultured microbes. Therefore, nucleic-acid-based approaches have been introduced to study microbial diversity and activity, and these depend on the proper isolation of DNA, rRNA and mRNA.
Erwin G Zoetendal et al.
Nature protocols, 1(2), 870-873 (2007-04-05)
The human gastrointestinal (GI) tract contains a complex microbial community that develops in time and space. The most widely used approaches to study microbial diversity and activity are all based on the analysis of nucleic acids, DNA, rRNA and mRNA.
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Dalton transactions (Cambridge, England : 2003), 43(31), 11941-11949 (2014-06-27)
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