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CRM12821M

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Legionella pneumophila NCTC 12821 Lenticule® discs

500-50000 CFU, suitable for microbiology, certified reference material, Manufactured by: Sigma-Aldrich Production GmbH, Switzerland

Synonym(s):

L. pneumophila NCTC 12821 Lenticule® discs, Legionella pneumophila NCTC 12821 Lenticule® discs, Certified reference material, Microbiological standards

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About This Item

UNSPSC Code:
41116107
NACRES:
NA.24

grade

certified reference material

Quality Level

agency

ISO 17034
ISO/IEC 17025

form

disc (hydrosoluble)
solid

shelf life

limited shelf life, expiry date on the label

packaging

pack of 10

manufacturer/tradename

Manufactured by: Sigma-Aldrich Production GmbH, Switzerland

application(s)

environmental
food and beverages

microbiology

storage temp.

−20°C

suitability

Legionella pneumophila
Legionella spp.
Legionella spp.

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General description

Legionella pneumophila NCTC 12821 Lenticule® discs are Certified Microorganisms Standards (Certified Microbiological Reference Materials), which can be used as quantitative and qualitative control organisms. They contain colony forming units (CFUs) in the range of 500-50000 CFU per disc.

The technology allows long-term conservation and renders the organisms resistant to temperature changes. They are intended to be used as daily internal quality control organisms.

These microorganisms are certified according to ISO/IEC 17025 and produced under reproducible conditions compliant with ISO 17034 using authenticated strains from NCTC. These ready-to-use microbiological controls minimize the need for maintaining control strains in the test laboratory and guarantee the use of an authenticated control culture for every quality control test.

Lenticule® discs are prepared from a traceable culture obtained freeze-dried from the National Collection of Type Cultures (NCTC) or National Collection of Pathogenic Fungi (NCPF) and are manufactured by Sigma-Aldrich under license from the UK Health Security Agency.

Application

Legionella pneumophila NCTC 12821 Lenticule® discs are used as reference strains in routine quality controlfor food, beverage, water, and environmental testing. Additionally, they areexcellent for use in method development and validations, as starter cultures, andfor use in research studies and educational purposes.

Features and Benefits

  • Ready-to-use at convenient CFU ranges
  • No dilution required
  • Quick rehydration in 10 minutes
  • At least 16-month shelf life in a standard -20 °C freezer
  • Complete with a comprehensive certificate of analysis
  • No maintenance of stock cultures
  • No recovery time or pre-enrichment step is necessary
  • Robust, efficient, and reliable
  • Easy-to-use
  • Cost-effective

Other Notes

Strain origin: NCTC 12821

Other cell culture collections:
  • DSM 27564
  • CECT 8734
Click here to access the BacDive database for more info on metabolism, genome, growth media, and more
Strain History - Accessioned into the NCTC in 1993 < Isolated by Dr J. Lee, from Water, Queens Medical Centre Nottingham UK, in 1993.

Legal Information

Lenticule is a registered trademark of The Secretary of State for Health (United Kingdom)

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3


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The use of LenticulesTM for the process control of enumeration techniques in food and environmental microbiology.
Lightfoot N F, et al.
Journal of Applied Microbiology, 91(4), 660-667 (2001)

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