Hoechst 33342 can be dissolved in N,N-dimethylformamide (DMF).
B2261
bisBenzimide H 33342 trihydrochloride
≥98% purity (HPLC and TLC), powder
Synonym(s):
2′-(4-Ethoxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5′-bi-1H-benzimidazole trihydrochloride, HOE 33342, Hoechst 33342, bisBenzimide
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About This Item
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Product Name
bisBenzimide H 33342 trihydrochloride, ≥98% (HPLC and TLC)
Quality Level
assay
≥98% (HPLC and TLC)
form
powder
technique(s)
titration: suitable
color
yellow
pH
1.7 (20 °C)
solubility
H2O: 20 mg/mL
phosphate buffer: precipitates
suitability
suitable for fluorescence
application(s)
diagnostic assay manufacturing
hematology
histology
storage temp.
−20°C
SMILES string
Cl[H].Cl[H].Cl[H].CCOc1ccc(cc1)C2=NCc3cc(ccc3N2)C4=NCc5cc(ccc5N4)N6CCN(C)CC6
InChI
1S/C29H32N6O.3ClH/c1-3-36-25-8-4-20(5-9-25)28-30-18-22-16-21(6-10-26(22)32-28)29-31-19-23-17-24(7-11-27(23)33-29)35-14-12-34(2)13-15-35;;;/h4-11,16-17H,3,12-15,18-19H2,1-2H3,(H,30,32)(H,31,33);3*1H
InChI key
FYEVKHPLBHLWHK-UHFFFAOYSA-N
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Application
Excitation max. = 346 nm
Emission max. = 460 nm
Biochem/physiol Actions
Physical properties
Preparation Note
Aqueous solutions are stable for 1 month if kept in the dark at 2-8 °C.[3]
related product
Storage Class
11 - Combustible Solids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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Articles
Cell cycle regulates vital processes like DNA repair, cancer prevention. Four stages: G1, S, G2, M. NTPs don't permeate membranes.
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Can solvents other than water be used to dissolve Product B2261, bisBenzimide H 33342 trihydrochloride?
1 answer-
Helpful?
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
Helpful?
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What is a typical concentration of Product B2261, bisBenzimide H 33342 trihydrochloride, for staining DNA in cells?
1 answer-
200 micrograms per mL.
Helpful?
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Which Hoechst dye is suitable for chromosomal analysis?
1 answer-
Hoechst 33258 (Product No. B2883) is the preferred Hoechst dye for chromosomal analysis. Hoechst 33342 (Product No. B2261) is a poor fluorochrome for chromosomal analysis.
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