93630
Trypsin, TPCK treated, from bovine pancreas
~7500 U/mg
Synonym(s):
TPCK-Trypsin
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About This Item
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specific activity
~7500 U/mg
shipped in
wet ice
storage temp.
−20°C
Unit Definition
1 U corresponds to the amount of enzyme which increases the absorbance at 253 nm by 0.001 per minute at pH 7.6 and 25°C (N-benzoyl-L-arginine ethyl ester, Cat. No. 12880, as substrate)
Other Notes
Sales restrictions may apply
TPCK-treatment irreversibly inhibits the chymotrypsin-activity without affecting the trypsin-activity; Application in (selective) hydrolysis/condensation of amide bonds; Tryptic degradation of opioid peptide precursors in rat brain
signalword
Danger
hcodes
Hazard Classifications
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
target_organs
Respiratory system
Storage Class
11 - Combustible Solids
wgk_germany
WGK 1
ppe
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
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G Q Zhang et al.
Chemical & pharmaceutical bulletin, 39(9), 2369-2372 (1991-09-01)
A high-performance liquid chromatographic method involving postcolumn fluorescence derivatization is described for the quantification of five fragment peptides (methionine-enkephalin-Thr-Ser-Glu-Lys, methionine-enkephalin-Lys, methionine-enkephalin-Arg, leucine-enkephalin-Lys and leucine-enkephalin-Arg) released by tryptic digestion from the opioid peptide precursors (proopiomeranocortin and proenkephalins A and B) in
A. Florsheimer et al.
Monatshefte fur Chemie / Chemical Monthly, 119, 1323-1323 (1988)
Direct evidence for the presence of histidine in the active center of chymotrypsin.
G SCHOELLMANN et al.
Biochemistry, 2, 252-255 (1963-03-01)
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