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Millipore

Tetrathionate Broth

suitable for microbiology, NutriSelect® Plus

Synonym(s):

TB, TT Broth

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About This Item

UNSPSC Code:
41171607
NACRES:
NA.74

sterility

non-sterile

Quality Level

200

form

powder

shelf life

limited shelf life, expiry date on the label

composition

bile salts, 1 g/L
calcium carbonate, 10 g/L
mixed peptone, 5 g/L
sodium thiosulfate, 30 g/L

manufacturer/tradename

NutriSelect® Plus

technique(s)

microbiological culture: suitable

final pH

7.8±0.2 (25 °C)

application(s)

environmental
food and beverages
pathogen testing
sample preparation
microbiology

suitability

selective for Salmonella spp.

Application

For the selective enrichment of Salmonella from various sources.

Preparation Note

Dissolve 46 g in 1 litre distilled water and heat carefully till boiling (do NOT sterilize). Cool to below 45°C then add 20 ml iodide solution (5 g potassium iodide (Cat. No. 60400) and 6 g iodine (Cat. No. 57650) in 20 ml distilled water) and 10 ml 0.1% brilliant green (Cat. No. 16020; 10 mg/l) solution. Mix and fill containers so that any insoluble material is evenly distributed. This broth should be prepared on the day of use.

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

13 - Non Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
BCCD4862
BCBW4852
BCBW4851
BCBR5655V
BCBR5656V

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L. Muller
Comp. rend. Soc. biol., 89, 434-434 (1923)
Novobiocin-tetrathionate broth: a medium of improved selectivity for the isolation of Salmonellae from faeces.
L JEFFRIES
Journal of clinical pathology, 12, 568-571 (1959-11-01)
Stefanie Mangold et al.
Frontiers in microbiology, 2, 17-17 (2011-06-21)
Given the challenges to life at low pH, an analysis of inorganic sulfur compound (ISC) oxidation was initiated in the chemolithoautotrophic extremophile Acidithiobacillus caldus. A. caldus is able to metabolize elemental sulfur and a broad range of ISCs. It has
S A Palumbo et al.
Applied microbiology, 20(6), 970-976 (1970-12-01)
Tetrathionate enrichment broth is a complex mixture of salts including iodides and other polythionates, but only thiosulfate (0.0736 m) and tetrathionate (0.0236 m) in combination were toxic for Escherichia coli. Individually, these two salts were not lethal. The lethal action
Kauffmann, F.
Zentralblatt fur Bakteriologie, Parasitenkunde, Infektionskrankheiten Und Hygiene. 1. Abt. Medizinisch-Hygienische Bakteriologie, Virusforschung und Parasitologie. Originale, 117, 26-26 (1935)

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