As mentioned on the website, under 'Preparation Note', dissolve 10.5 g in 1 litre distilled water and adjust the pH to 7.4. Sterilize by autoclaving at 121°C for 15 minutes. Add 2 ml 1 M magnesium sulfate (Cat. No. 63133; filter sterilized) and 20 ml of 20% solution from a carbon source (such as glucose; Cat. No. 49140).
63011
M9 Broth
suitable for microbiology, NutriSelect® Basic
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500 G
$348.00
About This Item
UNSPSC Code:
41171607
NACRES:
NA.74
Recommended Products
sterility
non-sterile
Quality Level
form
powder
composition
ammonium chloride, 1 g/L
disodium hydrogen phosphate, 6 g/L
potassium dihydrogen phosphate, 3 g/L
sodium chloride, 0.5 g/L
manufacturer/tradename
NutriSelect® Basic
technique(s)
microbiological culture: suitable
final pH
7.4±0.2 (25 °C)
application(s)
food and beverages
life science and biopharma
microbiology
suitability
nonselective for Escherichia coli
nonselective for coliforms
Related Categories
Application
M9 Broth is used as a microbiological growth media and for the preparation of phage and plasmid DNA using E. coli K12 strains, acc. to Miller (1987).
Preparation Note
Dissolve 10.5 g in 1 litre distilled water and adjust the pH to 7.4. Sterilize by autoclaving at 121°C for 15 minutes. Add 2 ml 1 M magnesium sulfate (Cat. No. 63133; filter sterilized) and 20 ml of 20% solution from a carbon source (such as glucose; Cat. No. 49140)
Footnote
We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.
Legal Information
GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class
11 - Combustible Solids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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