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Millipore

ONPG Disks

suitable for microbiology

Synonym(s):

β-Galactosidase Test Disks, 2-Nitrophenyl-β-D-galactopyranoside Disks

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About This Item

UNSPSC Code:
41171621
NACRES:
NA.85

agency

according to GB 4789.4-2016

product line

BioChemika

shelf life

limited shelf life, expiry date on the label

technique(s)

microbe id | specific enzyme detection: suitable

application(s)

environmental
food and beverages
microbiology

shipped in

wet ice

storage temp.

2-8°C

suitability

Escherichia coli
bacteria

General description

ONPG Disks are used to detect the presence of β-galactosidase, an enzyme found in lactose-fermenting organisms. Lactose utilization depends upon two enzymes: β-galactoside permease, which catalyzes transport of lactose into the cell, and β-galactosidase, which breaks down lactose into galactose and glucose. β-Galactosidase is not lactose specific and can act on simple galactosides including the ONPG (o-nitrophenyl-β-D-galactopyranose) substrate. ONPG hydrolysis results in the release of galactose, and the yellow chromogenic compound, o-nitrophenol. The test substrate, ONPG, does not depend on an induced or constitutive permease enzyme to enter the cell, therefore reactions are rapid and occur within a 24-hour period even for late lactose fermenters.

Packaging

Sterile filter paper discs (50 pcs, diameter 6 mm) impregnated with o-nitrophenyl-β-D-galactopyranose

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
BCCL6201
BCCM1925
BCCK3268
BCCJ4470
BCCG9930

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Leann Clark et al.
Microbiology (Reading, England), 157(Pt 7), 2072-2083 (2011-04-16)
Most studies on Salmonella enterica serovar Typhimurium infection focus on strains ATCC SL1344 or NTCC 12023 (ATCC 14028). We have compared the abilities of these strains to induce membrane ruffles and invade epithelial cells. S. Typhimurium strain 12023 is less

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