06105

Bile esculin azide agar

suitable for microbiology, NutriSelect^® Plus

• Synonym(s): Enterococcus Selective Agar
• UNSPSC Code: 41171606
• NACRES: NA.85

Properties

• sterility: non-sterile
• Quality Level: 200
• form: powder
• shelf life: limited shelf life, expiry date on the label
• composition: agar, 15.0 g/L ; beef extract, 5.0 g/L ; casein enzymic hydrolysate, 17.0 g/L ; esculin, 1.0 g/L ; ferric ammonium citrate, 0.5 g/L ; oxgall, 10.0 g/L ; proteose peptone, 3.0 g/L ; sodium azide, 0.15 g/L ; sodium chloride, 5.0 g/L
• manufacturer/tradename: NutriSelect^® Plus
• technique(s): microbe id | specific enzyme detection: suitable
• application(s): clinical testing; environmental; food and beverages; water monitoring; microbiology
• suitability: selective and differential for Enterococcus spp.; selective and differential for Streptococcus spp.

Description

General description

Bile esculin agar (BEA) is a differential and selective medium and is mainly used to discern group D Streptococci and Enterococci based on the organism′s potential to hydrolyze esculin. BEA comprises of oxygall (bile salts, first selective ingredients) and azide (second selective ingredients), former inhibits the growth of gram positive organisms and latter inhibits the growth of gram negative organisms. BEA also comprises of nutrients ferric citrate and esculin, where esculin is a differential ingredient and is also a fluorescent compound, upon hydrolyzation the fluorescence is lost. BEA is used for bile esculin test which is based on the hydrolysis of esculin into esculetin (6, 7-dihydroxy-coumarin) and glucose by a micro-organism that produce an enzyme esculinase.

Application

Bile Esculin Azide Agar is a selective medium used for isolation and presumptive identification of faecal Streptococci.

Preparation Note

Suspend 56.65g in 1 l distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 121°C for 15 minutes.

Footnote

We offer two media types: the superior granulated GranuCult^® and the cost-efficient powdered NutriSelect^® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• hcodes: H412
• pcodes: P273 - P501
• Hazard Classifications: Aquatic Chronic 3
• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: dust mask type N95 (US), Eyeshields, Gloves