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DNALIG-RO

Roche

T4 DNA Ligase

solution, from Escherichia coli(NM 989), suitable for activity assay

Synonym(s):

dna ligase, t4

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Pack SizeSKUAvailabilityPrice
100 units

Available to ship TODAYfromMILWAUKEE

$103.00
500 units

Available to ship TODAYfromMILWAUKEE

$327.00
500 units

Available to ship TODAYfromMILWAUKEE

$335.00

About This Item

UNSPSC Code:
12352204
NACRES:
NA.54
Specific activity:
~3000 units/mg protein
Biological source:
Escherichia coli (NM 989)
Recombinant:
expressed in E. coli

$103.00

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biological source

Escherichia coli (NM 989)

recombinant

expressed in E. coli

description

recombinant form of the enzyme from T4 phage

form

solution

specific activity

~3000 units/mg protein

packaging

pkg of 100 U (10481220001 [1 U/μl]), pkg of 500 U (10716359001 [1 U/μl]), pkg of 500 U (10799009001 [5 U/μl])

manufacturer/tradename

Roche

parameter

4-22 °C optimum reaction temp.

technique(s)

activity assay: suitable

optimum pH

7.2-7.8

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This Item
GE27-0361-0170099-MD2886
T4 DNA Ligase solution, from Escherichia coli(NM 989), suitable for activity assay

Roche

DNALIG-RO

T4 DNA Ligase

Ready-to-go™ T4 DNA Ligase Cytiva 27-0361-01, sufficient for 50 reactions

GE27-0361-01

Ready-to-go™ T4 DNA Ligase

T4 DNA Polymerase, LIC-qualified - Novagen Novagen′s T4 DNA Polymerase has been qualified for ligation-independent cloning using a unique assay to verify its performance.

Sigma-Aldrich

70099-M

T4 DNA Polymerase, LIC-qualified - Novagen

DNA Ligase from T4-infected Escherichia coli buffered aqueous glycerol solution

Sigma-Aldrich

D2886

DNA Ligase from T4-infected Escherichia coli

biological source

Escherichia coli (NM 989)

biological source

-

biological source

-

biological source

-

technique(s)

activity assay: suitable

technique(s)

-

technique(s)

molecular cloning: suitable

technique(s)

-

specific activity

~3000 units/mg protein

specific activity

-

specific activity

-

specific activity

4,000 U/mL

form

solution

form

-

form

liquid

form

buffered aqueous glycerol solution

manufacturer/tradename

Roche

manufacturer/tradename

Cytiva 27-0361-01

manufacturer/tradename

Novagen®

manufacturer/tradename

-

storage temp.

−20°C

storage temp.

-

storage temp.

−20°C

storage temp.

−20°C

General description

T4 DNA Ligase catalyzes the formation of phosphodiester bonds between neighboring 3′-hydroxyl- and 5′-phosphate ends in double-stranded DNA. Single-stranded nicks in double-stranded DNA are also closed by T4 DNA Ligase.

Contents
T4 DNA Ligase, supplied with 10x concentrated ligation buffer that includes ATP.

Application

Ligation of sticky- and blunt-ended DNA fragments.[1][2][3]

Biochem/physiol Actions

Heat inactivation: The enzyme can be inactivated by heating (65 °C, 10 minutes).

Preparation Note

Activator: Mg2+ (10mM), DTT
Working solution: For dilution of the enzyme Roche recommends using a buffer containing the components of the storage buffer: 20 mM Tris-HCl, 60 mM KCl, 1 mM EDTA, 5 mM dithoerythritol, 50% glycerol (v/v), pH 7.5 (4 °C).

Analysis Note

Quality

Tested for the absence of deoxyribonucleases and exonucleases, according to the current Quality Control procedures.

Other Notes

For life science research only. Not for use in diagnostic procedures.
One unit of T4 DNA ligase is the amount of enzyme activity that converts 1 nmol 32P from pyrophosphate into Norit-absorbable material in 20 minutes at +37 °C. One unit corresponds to 0.2 U determined in the exonuclease III resistance assay. Dilution buffer: 50 mM Tris-HCl, 10 mM dithioerythritol, bovine serum albumin, 500 μg/ml, pH 7.6 (+25 °C).

Volume Activity: 1 x 103 U/ml; 5 x 103 U/ml

Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

does not flash

flash_point_c

does not flash

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Certificates of Analysis (COA)

Lot/Batch Number
67371424
67371423
64436921
53744223
53744222

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Global Trade Item Number

SKUGTIN
1071635900104061837684500
1048122000104061838672094
1079900900104061837684517

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