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Roche

Biotin-16-UTP

pkg of 25 μL (250 nmol; 10mM)

Synonym(s):

Biotin-16-UTP, biotin

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About This Item

UNSPSC Code:
41116100

assay

97.7% (HPLC)

Quality Level

100

form

solution

mol wt

Mr 987.5 (biotin-16-UTP-Li4)

packaging

pkg of 25 μL (250 nmol; 10mM)

manufacturer/tradename

Roche

color

colorless

solubility

water: miscible

shipped in

dry ice

storage temp.

−20°C

General description

For biotin-16-UTP, biotin is bound to uridine triphosphate via an amide linkage. Biotin-16-UTP is a substrate for SP6, T3, and T7 RNA polymerase. It can replace UTP in the in vitro transcription reaction for RNA labeling. Linearized template DNA with T7, SP6, or T3 promoter is in vitro-transcribed with the corresponding RNA polymerases using ATP, GTP, CTP, UTP, and Biotin-16-UTP, respectively.

Application

Biotin-labeled RNA can be used as a hybridization probe for:
  • Northern blots
  • Southern blots
  • Plaque or colony lifts
  • RNase protection experiments
  • In situ hybridization
  • Microarray hybridization
The labeled RNA can be subsequently detected with a fluorescent streptavidin conjugate or by enzyme linked immuno sorbent assay (ELISA) using a Streptavidin-AP conjugate.

Quality

Typical analysis: At least 85% Biotin-16-UTP (HPLC, area%).

Physical form

Biotin-16-UTP, tetralithium salt, 10mM solution

Other Notes

For life science research only. Not for use in diagnostic procedures.

wgk_germany

WGK 1

flash_point_f

does not flash

flash_point_c

does not flash

Certificates of Analysis (COA)

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