SCT277

BioTracker^™ Red pH-Independent Autophagy Live Cell Probe

Human

• Synonym(s): autophagosome live cell probe, red AP live cell probe, red autophagosome probe, red autophagy probe
• UNSPSC Code: 41106514
• NACRES: NA.47

Properties

• product name: BioTracker^™ Red pH-Independent Autophagy Live Cell Probe,
• biological source: human
• Quality Level: 100
• packaging: vial of 5 nmol
• manufacturer/tradename: Millipore
• technique(s): cell culture | stem cell: suitable; flow cytometry: suitable
• shipped in: 2-8°C
• storage temp.: 2-8°C

Description

General description

The small fluorescent molecule SCT277 is used to detect autophagosomes and autolysosomes. The presumed mechanism is that the dye is incorporated into the autophagosome during double-membrane formation via structural features, and then emits fluorescence under hydrophobic conditions. The probe emits in the far red range.SPECTRAL CHARACTERISTICS: Excitation λ: 530 nmEmission λ: 720 nm

Features and Benefits

Fluorescence microscopyFlow cytometry

Target description

BioTracker Red pH-Independent Autophagy Live Cell Probe detects autophagosomes and autolysosomes. The presumptive mechanism involves incorporation of the probe via structural features into the autophagosome during double-membrane formation. Upon incorporation, the probe emits fluorescence under hydrophobic conditions. The utility of the probe is conferred by molecular properties that include cell permeability, does not require transfection, and enables live cell detection with fluorescence microscopy or other fluorescence capture instruments.

Storage and Stability

Store at -20 °C, protected from light.

Other Notes

SCT277 detects autophagosomes and autolysosomes by the likely mechanism of incorporation into the autophagosome during double membrane formation, and emits red fluorescence under hydrophobic conditions. SCT277 is cell permeable, does not require transfection, and enables live cell imaging with fluorescence microscopy and quantitative assay by flow cytometry.Add 50 uL DMSO to tube of SCT277 (5 nmol) and pipet to dissolve to make a 0.1 mM stock solution. Dilute with culture medium to 0.1 - 0.5 uM to make working solution.

Legal Information

BioTracker is a trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable