SCT059

BioTracker TiY Vimentin Live Cell Dye

Live cell imaging probe for identifying tumor initiating cells (TICs) in a variety of epithelial cancers, including lung cancer. The TiY probe binds to vimentin expressed in TICs.

• Synonym(s): Live cell imaging probe, Tumor Initiating Cell (TIC) dye
• NACRES: NA.47
• UNSPSC Code: 12352207

Properties

• assay: ≥98% (H-NMR), ≥98% (HPLC), ≥98% (LC/MS), ≥98% (elemental analysis)
• technique(s): cell based assay: suitable
• detection method: fluorometric

Description

General description

Vimentin is an intermediate filament protein which is upregulated in cells undergoing epithelial-to-mesenchymal transition (EMT), and hence is commonly used an EMT marker. Tumor initiating cells (TICs) have been implicated in clinical relapse and metastasis of a variety of epithelial cancers, including lung cancer.

BioTracker TiY Vimentin is a live cell fluorescent dye that selectively stains TICs over differentiated tumor cells or normal cells. TiY can be used for visualization and enrichment of functionally active TICs from patient tumors. TiY displays better selectivity for tumorigenic cells than a CD166 antibody and can be used for TiY-staining-based cell sorting for TICs in various types of cancer (lung, CNS, melanoma, breast, renal, ovarian, colon and prostate cancer). At high concentrations, TiY shows selective anti-TIC activity over non-TIC and normal tissue cells.

Spectral Properties
Absorbance: 553 nm
Emission: 573 nm

References
Lee Y et al. Identification of Tumor Initiating Cells with a Small-Molecule Fluorescent Probe by Using Vimentin as a Biomarker. Angew Chem Int Ed Engl. 2018 Mar 5;57(11):2851-2854.

Application

Live cell fluorescent imaging

Live cell imaging probe for identifying tumor initiating cells (TICs) in a variety of epithelial cancers, including lung cancer. The TiY probe binds to vimentin expressed in TICs.

Research Category
Cell Imaging

Research Sub Category
Live Cell Dye

Physical form

Lyophilized

Preparation Note

Store BioTracker TiY Vimentin Live Cell Dye at -20°C, desiccate and protect from light
Note: Centrifuge vial briefly to collect contents at bottom of vial before opening.

Analysis Note

Purity: ≥ 98% confirmed by HNMR, LC-MS and HPLC and elemental analysis
Molar Mass: 541.87 g/mol

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable