MABS1931

Anti-CD368/CLEC4D Antibody, clone WEN42

• Synonym(s): C-type lectin domain family 4 member D (UniProt: Q69FH1, also known as C-type lectin superfamily member 8
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 200
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: WEN42, monoclonal
• mol wt: observed mol wt ~55 kDa
• purified by: using protein G
• species reactivity: rat
• packaging: antibody small pack of 100
• technique(s): flow cytometry: suitable; immunohistochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• isotype: IgG2a
• epitope sequence: Extracellular domain
• Protein ID accession no.: NP_001003707
• UniProt accession no.: Q69FH1
• storage temp.: 2-8°C
• Gene Information: rat ... Clec4d(362432)

Description

Specificity

Clone WEN42 is a mouse monoclonal antibody that detects C-type lectin domain family 4 member D (CD368/CLEC4D). It targets an epitope within the extracellular domain.

Immunogen

BWZ.36 cells expressing chimeric CD368/CLEC4D

Application

Quality Control Testing

Evaluated by Western Blotting with recombinant Rat CD368/CLEC4D protein.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected recombinant fragment of Rat CD368/CLEC4D expressed with Fc region of human IgG1.

Tested Applications

Immunoprecipitation Analysis: A representative lot immunoprecipitated CD368/CLEC4D in Immunoprecipitation applications (Lobato-Pascual, A., et al. (2013). PLoS One. 8(2):e57406; Lobato-Pascual, A., et al. (2013). Eur J Immunol. 43(12):3167-74).

Immunohistochemistry Applications: A representative lot detected CD368/CLEC4D in Immunohistochemistry applications (Lobato-Pascual, A., et al. (2013). PLoS One. 8(2):e57406).

Western Blotting Analysis: A representative lot detected CD368/CLEC4D in Western Blotting applications (Lobato-Pascual, A., et al. (2013). PLoS One. 8(2):e57406; Lobato-Pascual, A., et al. (2013). Eur J Immunol. 43(12):3167-74).

Flow Cytometry Analysis: A representative lot detected CD368/CLEC4D in Flow Cytometry applications (Lobato-Pascual, A., et al. (2013). PLoS One. 8(2):e57406; Lobato-Pascual, A., et al. (2013). Eur J Immunol. 43(12):3167-74).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Target description

C-type lectin domain family 4 member D (UniProt: Q69FH1; also known as C-type lectin superfamily member 8, CD368) is encoded by the Clec4d (also known as Clecsf8, Mcl) gene (Gene ID: 362432) in rat. CD368 is a membrane surface receptor that is expressed on blood monocytes and neutrophils, as well as on several tissue macrophage populations, including alveolar and peritoneal cavity macrophages. It is a single-pass type II protein that contains a cytoplasmic domain (aa 1-24), a transmembrane domain (aa 25-47), and an extracellular domain (aa 48-218). Its extracellular C-terminal region contains the C-type lectin-like domain (aa 89-206) that contains an evolutionarily conserved folded domain, and a carbohydrate recognition domain containing the Ca2+ binding sites. CD368 acts as a pattern recognition receptor (PRR) of the innate immune system that recognizes damage-associated molecular patterns (DAMPs) of pathogen-associated molecular patterns (PAMPs) of bacteria and fungi. It can form disulfide-linked heterodimer with C-type lectin domain family 4 member E (CLEC4E) and with CLEC6A that forms a PPR against fungal infections. (Ref.: Lobato-Pascual, A., et al. (2013). PLoS ONE 8(2); e57406; Lobato-pascual, A., et al. (2013). Eur. J. Immunol. 43(12); 3167-3174).

Physical form

Purified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Reconstitution

1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommended storage: +2°C to +8°C .

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable