MABN473

Anti-TMEM106B Antibody, clone TME-N 6F2

clone TME-N 6F2, from rat

• Synonym(s): Transmembrane protein 106B, TMEM106B
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: rat
• Quality Level: 100
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: TME-N 6F2, monoclonal
• species reactivity: rat, human
• technique(s): immunofluorescence: suitable; western blot: suitable
• NCBI accession no.: NP_001127704
• UniProt accession no.: Q9NUM4
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... TMEM106B(54664)

Description

General description

Transmembrane protein 106B (TMEM106B) is a single-pass type II membrane protein present in endosomal and lysosomal membranes, and is highly expressed in the frontal cortex. Increased TMEM106B expression in the brain may be linked to mechanisms of disease in frontotemporal lobar degeneration with TAR DNA-binding protein (TDP-43) inclusions (FTLD-TDP). Frontotemporal lobar degeneration (FTLD) is the second most common cause of presenile dementia.

Specificity

This antibody recognizes the N-terminus of TMEM106B.

Immunogen

Epitope: N-terminus

Recombinant protein corresponding to the N-terminus of human TMEM106B.

Application

Anti-TMEM106B, clone TME-N 6F2 detects levels of TMEM106B proteins & has been published & validated for use in WB & IF.

Immunofluorescence Analysis: A 1:50 dilution from a representative lot detected TMEM106B in rat cerebellum tissue.

Research Category
Neuroscience

Research Sub Category
Neurodegenerative Diseases

Quality

Evaluated by Western Blot in human cortex tissue lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected TMEM106B in 10 µg of human cortex tissue lysate.

Target description

~30 kDa observed (At higher antibody concentrations, this protein may be observed at ~45 kDa). This protein may be observed at ~50 kDa due to glycosylation (Lang, C. M., et al. (2012). J Biol Chem. 287(23):19355-19365.).

Physical form

Format: Purified

Protein G Purified

Purified rat monoclonal IgG2c in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Human cortex tissue lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable