MABN2592
Anti-RBP3 Antibody, clone mAb5
Synonym(s):
IRBP, Interphotoreceptor retinoid-binding protein, Interstitial retinol-binding protein, Protein 7S, Retinol-binding protein 3
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About This Item
UNSPSC Code:
12352203
biological source
mouse
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
mAb5, monoclonal
species reactivity
bovine
packaging
antibody small pack of 100
technique(s)
electron microscopy: suitable
immunoprecipitation (IP): suitable
western blot: suitable
isotype
IgG1κ
epitope sequence
N-terminal half
Protein ID accession no.
UniProt accession no.
Gene Information
bovine ... RBP3(281443)
Specificity
Clone mAb5 is a mouse monoclonal antibody that detects Retinol-binding protein 3 (RBP3). It targets an epitope within the N-terminal half.
Immunogen
Purified Retinol-binding protein 3 (RBP3) from bovine retina.
Application
Quality Control Testing
Evaluated by Western Blotting in lysate from Bovine retinal tissue.
Western Blotting Analysis: A 1:500 dilution of this antibody RBP3 in lysate from Bovine retinal tissue.
Tested Applications
Electron Microscopy: A representative lot detected RBP3 in Electron Microscopy applications (Sears, A.E., et al. (2020). FASEB J. 34(10):13918-13934).
Immunoprecipitation Analysis: A representative lot immunoprecipitated RBP3 in Immunoprecipitation applications (Sears, A.E., et al. (2020). FASEB J. 34(10):13918-13934).
Western Blotting Analysis: A representative lot detected RBP3 in Western Blotting applications (Sears, A.E., et al. (2020). FASEB J. 34(10):13918-13934).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Evaluated by Western Blotting in lysate from Bovine retinal tissue.
Western Blotting Analysis: A 1:500 dilution of this antibody RBP3 in lysate from Bovine retinal tissue.
Tested Applications
Electron Microscopy: A representative lot detected RBP3 in Electron Microscopy applications (Sears, A.E., et al. (2020). FASEB J. 34(10):13918-13934).
Immunoprecipitation Analysis: A representative lot immunoprecipitated RBP3 in Immunoprecipitation applications (Sears, A.E., et al. (2020). FASEB J. 34(10):13918-13934).
Western Blotting Analysis: A representative lot detected RBP3 in Western Blotting applications (Sears, A.E., et al. (2020). FASEB J. 34(10):13918-13934).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Target description
Retinol-binding protein 3 (UniProt: P12661; also known as Interphotoreceptor retinoid-binding protein, IRBP, Interstitial retinol-binding protein, RBP3, Protein 7S) is encoded by the RBP3 gene (Gene ID: 281443) in bovine. RBP3 is synthesized with a signal peptide (aa 1-17) and a propeptide (aa 18-22) that are subsequently cleaved off to produce the mature form. It is a lipophilic glycoprotein that is secreted by rod and cone photoreceptors and plays a major role in photoreceptor homeostasis as well as retinoid and polyunsaturated fatty acid transport between the neural retina and retinal pigment epithelium. Its expression is observed early in retinal differentiation. RBP3 shuttles 11-cis and all trans retinoids between the retinol isomerase in the pigment epithelium and the visual pigments in the photoreceptor cells of the retina. It can bind many retinoids and polyunsaturated fatty acids and in addition to retinoid binding, it also plays a role in retinal development and homeostasis. RBP3 also displays thiol-dependent antioxidant activity that maintains the delicate redox balance in the normal retina. RBP3 dysregulation has been linked to retinal diseases. Mice lacking RBP3 exhibit severe early and progressive photoreceptor degeneration that is characterized by a reduction in both length and numbers of cone sheaths. In mammals four protein subunits of RBP3 have been described (M1-4), each consisting of about 300 amino acids. Clone mAb5 has been mapped to M2 subunit of RBP3. (Ref.: Sears, AE., et al. (2020) FASEB J. 34(10); 13918-13934; Zheng, S., et al. (2020). Front. Cell. Neurosci. 14; Article 577935).
Physical form
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl and 0.05% sodium azide.
Reconstitution
0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Storage and Stability
Recommended storage: +2°C to +8°C.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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