MABF505

Anti-CAMK4, clone 8C5B8 Antibody

clone 8C5B8, from mouse

• Synonym(s): Calcium/calmodulin-dependent protein kinase type IV, CaMK IV, CAMK4
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 100
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 8C5B8, monoclonal
• species reactivity: human
• technique(s): ELISA: suitable; immunofluorescence: suitable; immunohistochemistry: suitable; western blot: suitable
• isotype: IgG1
• UniProt accession no.: Q16566
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... CAMK4(814)

Description

General description

Calcium/calmodulin-dependent protein kinase type IV (CaMK IV), also known as CaM kinase-GR, and encoded by the gene name CAMK4 or CAMK or CAMK-GR or CAMKIV, is a multifunctional serine/threonine protein kinase with limited tissue distribution, that has been implicated in transcriptional regulation in lymphocytes, neurons and male germ cells. CAMK4 is localized in hippocampal neuron nuclei and is associated with chromatin and the nuclear matrix in spermatids. Expression in CAMK4 can be found in brain, thymus, CD4 T-cells, testis, and epithelial ovarian cancer tissue. CAMK4 can be phosphorylated by CaMKK1 and CaMKK2 on Thr200 and dephosphorylated by protein phosphatase 2A.

Immunogen

Purified recombinant fragment of human CAMK4 expressed in E. Coli

Application

Immunohistochemistry Analysis: A 1:200-1,000 dilution from a representative lot detected CAMK4 in ovarian and rectum cancer tissues.

Immunofluorescence Analysis: A 1:200-1,000 dilution from a representative lot detected CAMK4 in HepG2 cells.

ELISA: A representative lot detected control Antigen (100 ng) and Antigen (10 ng, 50 ng, 100 ng) in ELISA (Direct).

Research Category
Inflammation & Immunology

Research Sub Category
Immunoglobulins & Immunology

This Anti-CAMK4, clone 8C5B8 Antibody is validated for use in western blotting, IHC, immunofluorescence & ELISA for the detection of CAMK4.

Quality

Evaluated by Western Blotting in SK-N-SH, Raji and HeLa cell lysates.

Western Blotting Analysis: A 1:500-2,000 dilution of this antibody detected CAMK4 in SK-N-SH, Raji and HeLa cell lysates.

Target description

~57 kDa observed. Uncharacterized bands may be observed in some lysate(s).

Physical form

Format: Purified

Protein G Purified

Purified mouse monoclonal in PBS containing up to 0.1% sodium azide and 0.5% protein stabilizer.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable