MABF2008
Anti-IL-17F Antibody, clone MM17F-8F5
Synonym(s):
Interleukin-17F
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.43
Recommended Products
biological source
mouse
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
MM17F-8F5, monoclonal
mol wt
calculated mol wt 17.94 kDa
observed mol wt ~15 kDa
purified by
using protein G
species reactivity
mouse
packaging
antibody small pack of 100 μg
technique(s)
ELISA: suitable
flow cytometry: suitable
inhibition assay: suitable
neutralization: suitable
western blot: suitable
isotype
IgG1κ
epitope sequence
Unknown
Protein ID accession no.
UniProt accession no.
storage temp.
2-8°C
target post-translational modification
unmodified
Gene Information
mouse ... Il17f(257630)
General description
Interleukin-17F (UniProt: Q7TNI7; also known as IL-17F) is encoded by the Il17f gene (Gene ID: 257630) in murine species. The IL-17 family of cytokines contains six members that share four conserved cysteine residues at their C-terminal region that participate in the formation of intermolecular disulfide linkages. Members of this family are associated with the pathogenesis of multiple autoimmune diseases including rheumatoid arthritis, multiple sclerosis, and inflammatory bowel diseases. IL-17F is an effector cytokine of innate and adaptive immune system involved in antimicrobial host defense and maintenance of tissue integrity. It is a disulfide-linked homodimeric protein that is synthesized with a signal peptide (aa 1-28), which is subsequently cleaved off to produce mature secreted protein (aa 29-161). It can also form a heterodimer with IL-17A. Its actions are mediated via interaction with IL-17RA and IL-17RC receptors with 2:1 binding stoichiometry. It is primarily involved in host defense against extracellular bacteria and fungi by inducing neutrophilic inflammation. As signature effector cytokine of T-helper 17 cells (Th17), it induces neutrophil activation and recruitment at infection and inflammatory sites. It is also known to stimulate the production of antimicrobial β-defensins by mucosal epithelial cells thereby limiting the entry of microbes through the epithelial barriers. (Ref.: St. Leger, AJ., et al. (2017). Immunity 47(1); 148 158; Chang, SH., and Dong, C. (2009). Cytokine.46(1); 7-11).
Specificity
Clone MM17F-8F5 is a mouse monoclonal antibody that detects Interleukin-17F.
Immunogen
Ovalbumin-conjugated, full-length recombinant murine IL-17F.
Application
Quality Control Testing
Evaluated by Western Blotting with recombinant mouse IL-17F.
Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected recombinant mouse IL-17F.
Tested Applications
Flow Cytometry Analysis: A representative lot detected Interleukin-17F in Flow Cytometry application (St. Leger, A.J., et al. (2017). Immunity. 47(1):148-158.e5).
ELISA Analysis: Various dilutions of this antibody detected recombinant mouse IL-17F.
Neutralizing: A representative lot neutralized IL-17F activity and reduced neutrophil chemotactic activity produced by IL-17A / splenocytes activated under Th17 conditions.(Uyttenhove, C., et al. (2011). J Leukoc Biol. 89(6):1001-7).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Evaluated by Western Blotting with recombinant mouse IL-17F.
Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected recombinant mouse IL-17F.
Tested Applications
Flow Cytometry Analysis: A representative lot detected Interleukin-17F in Flow Cytometry application (St. Leger, A.J., et al. (2017). Immunity. 47(1):148-158.e5).
ELISA Analysis: Various dilutions of this antibody detected recombinant mouse IL-17F.
Neutralizing: A representative lot neutralized IL-17F activity and reduced neutrophil chemotactic activity produced by IL-17A / splenocytes activated under Th17 conditions.(Uyttenhove, C., et al. (2011). J Leukoc Biol. 89(6):1001-7).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Physical form
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Reconstitution
1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Storage and Stability
Recommended storage: +2°C to +8°C.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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