MABE339

Anti-p53 (wild type) Antibody, clone PAb1620

clone Pab1620, from mouse

• Synonym(s): Cellular tumor antigen p53, Tumor suppressor p53
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: Pab1620, monoclonal
• Application: IHC, WB
• Citations: 18

Properties

• biological source: mouse
• Quality Segment: 100
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: Pab1620, monoclonal
• species reactivity: human, mouse
• technique(s): immunohistochemistry: suitable, western blot: suitable
• isotype: IgG2aκ
• NCBI accession no.: NP_001120705
• UniProt accession no.: P02340
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... TP53(7157)

Description

General description

Wild-type p53 is present in the nucleus of all normal mammalian cells where it appears to be involved in the regulation of cell proliferation. The normal protein has a very short half-life and is present in only minute amounts in normal tissues and cells. In contrast, mutant p53 protein produced by malignant cells is usually a product of a point mutation in the p53 gene leading to substitution of a single amino acid that significantly prolongs the half-life of the protein. The accumulation of high levels of p53 is a potential novel marker for malignancy.

~53 kDa observed

Immunogen

Mouse VLM tumor cells.

Application

Anti-p53 (wild type) Antibody, clone PAb1620 is a Mouse Monoclonal Antibody for detection of p53 (wild type) also known as Cellular tumor antigen p53, Tumor suppressor p53 & has been validated in WB & IHC.

Immunohistochemistry Analysis: A 1:50 dilution of this antibody detected p53 in normal human brain tissue.

Immunoprecipitation Analysis: A representative lot from an independent laboratory detected p53 in IP (Lu, X., et al. (1992). Cell. 70(1):153-161.).

Immunohistochemistry Analysis: A representative lot from an independent laboratory detected p53 in dorsal skin samples from animal trunks (Kramata, P., et al. (2005). Cancer Res. 65(9):3577-3585.) and in certain human cancer tissues (Cordon-Cardo, C., et al, (1991). 51(23 Pt 1):6372-6380.).

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Cell Cycle, DNA Replication & Repair

Physical form

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Mouse brain tissue lysate

Evaluated by Western Blot in mouse brain tissue lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected p53 in mouse brain tissue lysate.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable