MABC1614

Anti-AATF Antibody, clone 1B2D8

from rat

• Synonym(s): Protein AATF, Apoptosis-antagonizing transcription factor, Rb-binding protein Che-1, Traube protein
• UNSPSC Code: 12352203
• eCl@ss: 32160702

Properties

• biological source: rat
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: monoclonal
• species reactivity: human, mouse
• packaging: antibody small pack of 25 μg
• technique(s): immunocytochemistry: suitable; western blot: suitable
• isotype: IgG2a
• UniProt accession no.: Q9JKX4
• target post-translational modification: unmodified
• Gene Information: human ... AATF(26574) ; mouse ... Aatf(56321)

Description

General description

Protein AATF (UniProt: Q9JKX4; also known as Apoptosis-antagonizing transcription factor, Rb-binding protein Che-1, Traube protein) is encoded by the Aatf (also known as Che1, Trb) gene (Gene ID: 56321) in murine species. AATF is a nuclear protein that functions as a general inhibitor of the histone deacetylase HDAC1. It binds to the pocket region of RB1 to displace HDAC1 from RB1/E2F complexes, leading to activation of E2F target genes and cell cycle progression. Conversely, displacement of HDAC1 from SP1 bound to the CDKN1A promoter leads to increased expression of this CDK inhibitor and blocks cell cycle progression. AATF is expressed in adrenal gland, Purkinje cells, heart, kidney, liver, lung, muscle, ovary, and testis. It is expressed uniformly throughout the embryo until E10.5. However, from E11.5, the relative expression level increases in the liver, hind brain, spinal cord, dorsal root ganglia, and the posterior commissure. Down-regulation of AATF is involved in colon carcinoma cell proliferation and over-expression of Aatf gene interferes with MAP3K12 induced apoptosis.

Specificity

Clone 1B2D8 is a rat monoclonal antibody that detects AATF in murine and human cells. It targets an epitope within the first 39 amino acids from the N-terminal region.

Immunogen

KLH-conjugated linear peptide corresponding to the first 39 amino acids from murine Apoptosis-antagonizing transcription factor (AATF).

Application

Detect AATF using this rat monoclonal Anti-AATF, clone 1B2D8 Antibody, Cat. No. MABC1614-25UG, validated for use in Western Blotting and Immunocytochemistry.

Immunocytochemistry Analysis: A representative lot detected AATF in Immunocytochemistry applications (Ishigaki, S., et. al. (2010). Cell Death Differ. 17(5):774-86).

Western Blotting Analysis: A representative lot detected AATF in Western Blotting applications (Ishigaki, S., et. al. (2010). Cell Death Differ. 17(5):774-86).

Research Category
Apoptosis & Cancer

Quality

Evaluated by Western Blotting in HeLa cell lysate. Western Blotting Analysis: 1 µg/mL of this antibody detected AATF in 10 µg of HeLa cell lysate.

Physical form

Format: Purified

Protein G purified

Purified rat monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable