MABC110

Anti-WIPI-2, clone 1B8.1 Antibody

clone 1B8.1, from mouse

• Synonym(s): WD repeat domain phosphoinositide-interacting protein 2, WIPI-2, WIPI49-like protein 2
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 100
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 1B8.1, monoclonal
• species reactivity: human
• technique(s): immunohistochemistry: suitable (paraffin); western blot: suitable
• isotype: IgG2aκ
• NCBI accession no.: NP_001028690
• UniProt accession no.: Q9Y4P8
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... WIPI2(26100)

Description

General description

WIPI-2, also known as Atg18, is involved in autophagy by regulating the assembly of mulitprotein complexes by presenting a 7-bladed propeller structure for interactions with phospholipids. Atg18 is a mammalian effector of PtdIns3P and is ubiquitously expressed in a variety of cell lines. Studies show that Atg18 is recruited to early autophagosomal structural formation along with Atg16 and ULK1, and Atg18 is required for the formation of LC3-positive autophagosomes.

Immunogen

Epitope: C-terminus

GST-tagged recombinant protein corresponding to the C-terminus of human WIPI-2.

Application

Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected WIPI-2 in human pancreas tissue.

Research Category
Apoptosis & Cancer

Research Sub Category
Apoptosis - Additional

This Anti-WIPI-2, clone 1B8.1 Antibody is validated for use in Western Blotting and Immunohistochemistry (Paraffin) for the detection of WIPI-2.

Quality

Evaluated by Western Blotting in human testis tissue lysate.

Western Blotting Analysis: 2.0 µg/mL of this antibody detected WIPI-2 in 10 µg of human testis tissue lysate.

Target description

~49 kDa observed. Uncharacterized band(s) may appear in some lysates.

Physical form

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable