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MAB4301C3

Sigma-Aldrich

Anti-SSEA1 Antibody, clone MC-480, Cy3 conjugate

clone MC-480, from mouse, CY3 conjugate

Synonym(s):

SSEA-1, SSEA1, CD15, Lewis X, 3-FAL

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

100

conjugate

CY3 conjugate

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

MC-480, monoclonal

species reactivity

human

technique(s)

immunocytochemistry: suitable

isotype

IgM

NCBI accession no.

NP_002024

UniProt accession no.

P22083

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... FUT4(2526)

General description

Stage-specific embryonic antigen-1 (SSEA-1) is expressed on the surface of early mouse embryos, murine embryonal carcinoma cells (EC), murine embryonic stem cells (ES) and murine & human germ cells (EG). No immunoreactivity is evident with undifferentiated human EC and ES cells. Expression of SSEA-1 is down regulated following differentiation of murine EC and ES cells. In contrast, the differentiation of human EC and ES cells is characterized by an increase in SSEA-1 expression.

Specificity

This antibody recognizes mouse Stage-Specific Embryonic Antigen-1 (SSEA-1).

Immunogen

F9 teratocarcinoma stem cells (X-irradiated).

Application

Detect SSEA1 using this Anti-SSEA1 Antibody, clone MC-480, Cy3 conjugate validated for use in ICC.
Evaluated by Immunocytochemistry in H9 human embryonic stem cells. Immunocytochemsitry Analysis: A 1:100 dilution of this antibody detected no SSEA-1 in H9 human embryonic stem cells.
Research Category
Stem Cell Research
Research Sub Category
Pluripotent & Early Differentiation

Glycobiology

Quality

Evaluated by Immunocytochemistry in mouse embryonic stem cells (SCR012). Immunocytochemsitry Analysis: A 1:100 dilution of this antibody detected SSEA-1 in mouse embryonic stem cells (SCR012).

Target description

The unconjugated parent antibody (Catalog No. MAB4301) has an observed MW at 220-240 kDa

Physical form

Purified mouse monoclonal IgM conjugated to Cy3 in PBS with 0.1% sodium azide and 15mg/ml BSA.

Storage and Stability

Maintain refrigerated at 2-8ºC protected from light in undiluted aliquots for up to 6 months from date of receipt.

Analysis Note

Control
Mouse embryonic stem cells (SCR012).

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Junyu Wu et al.
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Nucleic acid-based systems play important roles in antiviral defense, including CRISPR/Cas that adopts RNA-guided DNA cleavage to prevent DNA phage infection and RNA interference (RNAi) that employs RNA-guided RNA cleavage to defend against RNA virus infection. Here, we report a

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