MAB131871
Anti-KLH Antibody, clone 3B1
clone 3B1, from mouse
Synonym(s):
Keyhole Limpet Hemocyanin
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About This Item
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biological source
mouse
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
3B1, monoclonal
species reactivity
keyhole limpet, mollusk
technique(s)
western blot: suitable
isotype
IgMκ
shipped in
ambient
target post-translational modification
unmodified
General description
Substances with low molecular weights are generally not immunogenic and require the aid of carrier proteins to stimulate an immune response. Carrier proteins have relatively high molecular weights and carry many epitopes. An immune response to a hapten-carrier conjugate results in antibodies that are specific for both the hapten and the carrier. Hemocyanin is a high molecular weight respiratory metalloprotein found in the hemolymph of many mollusks and crustaceans. Molluscan hemocyanin is based on an approximately 400 kDa polypeptide subunit that is folded into a chain of multiple, immunologically distinct domains, each with a binuclear copper site. Keyhole Limpet Hemocyanin (KLH), the hemocyanin from the giant keyhole limpet, Megathura crenulata, is the most widely used carrier protein. It is an extremely large protein (6 - 7.5 million daltons) that can exist as a di-decamer, multi-decamers, or flexible tubules of varying length. It is easily dissociated into subunits and the dissociation-association reaction depends on protein concentration, ionic strength, and pH. At pH 6.5, KLH exists in a fully associated state without any evidence of dissociation products. In contrast, at pH 8.5 in borate saline the molecule is completely dissociated.
Specificity
Clone 3B1 detects keyhole limpet hemocyannin (KLH) and KLH conjugated to peptide or protein.
Immunogen
Purified Keyhole limpet hemocyanin from Megathura crenulata
Application
Anti-KLH, clone 3B1, Cat. No. MAB131871, is a mouse monoclonal antibody that detects Keyhole limpet hemocyanin and has been tested for use in Western Blotting.
Research Category
Inflammation & Immunology
Inflammation & Immunology
Quality
Evaluated by Western Blotting with Keyhole Limpet Hemocyanin (KLH).
Western Blotting Analysis: A 1:500 dilution of this antibody detected Keyhole Limpet Hemocyanin.
Western Blotting Analysis: A 1:500 dilution of this antibody detected Keyhole Limpet Hemocyanin.
Target description
~375 kDa observed; Calculated ~ 6 to 7.5 MDa (undissociated). Uncharacterized bands may be observed in some lysate(s).
Physical form
Format: Purified
Purified mouse monoclonal antibody IgM in PBS with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 2
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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