HTS089RTA
Ready-to-Assay LPA1 Lysophospholipid Receptor Frozen Cells
Human
About This Item
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Product Name
Ready-to-Assay LPA1 Lysophospholipid Receptor Frozen Cells, Human LPA1 / EDG2 GPCR frozen cells for Calcium Flux FLIPR Assays.
biological source
human
manufacturer/tradename
Ready-to-Assay
technique(s)
calcium flux assay: suitable
NCBI accession no.
detection method
fluorometric
shipped in
dry ice
General description
Lysophosphatidic acid (LPA) is a lysophospholipid produced by activated platelets that inhibits adenylate cyclase and stimulates DNA synthesis, changes in cell morphology, and increases in intracellular calcium in a variety of cultured mammalian cells. A family of three GPCRs, LPA1, LPA2 and LPA3, mediates the biological effects of LPA (Contos et al., 2000). LPA1 is widely expressed, with particularly notable expression in the ventricular zone of the embryonic cerebral cortex. Mice lacking LPA1 have changes in brain 5-HT and amino acids, and craniofacial abnormalities (Harrison et al., 2003). Millipore’s cloned human LPA1-expressing cell line is made in the Chem-1 host, which supports high levels of recombinant LPA1 expression on the cell surface and contains high levels of the promiscuous G protein Gα15 to couple the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for agonists, antagonists and modulators at LPA1.
Cell Line Description
Biochem/physiol Actions
Components
Fifty (50) mL of Media Component.
Storage Class
10 - Combustible liquids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
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