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Key Documents

FCMAB183F

Sigma-Aldrich

Milli-Mark® Anti-CD16-FITC Antibody, clone DJ130c

clone DJ130c, Milli-Mark®, from mouse

Synonym(s):

Low affinity immunoglobulin gamma Fc region receptor III-A, IgG Fc receptor III-2, Fc-gamma RIII-alpha, Fc-gamma RIIIa, Fc-gamma RIII, FcRIIIa, FcRIII, FcR-10, CD16a antigen

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.44

biological source

mouse

Quality Level

conjugate

FITC conjugate

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

DJ130c, monoclonal

species reactivity

human

manufacturer/tradename

Milli-Mark®

technique(s)

flow cytometry: suitable

isotype

IgG1κ

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... FCGR3A(2214)

General description

CD16 is a 50-70 kDa glycoprotein which occurs in two isoforms, CD16a and CD16b. CD16a is a transmembrane molecule expressed on about 90% of NK cells and also found on macrophages and subsets of monocytes and T cells. CD16b is glycosyl phosphatidyl inositol-anchored and is expressed on virtually all neutrophils (Tetteroo, 1987; Tamm, 1996). Anti-CD16, DJ130c, was included in the Fifth International Workshop and Conference on Human Leucocyte Differentiation Antigens, and studies by a number of laboratories confirmed its reactivity with CD16 (Schmidt, 1995).

Specificity

Antibody recognizes Human CD16 antigen.

Application

Detect CD16 using this Milli-Mark Anti-CD16-FITC Antibody, clone DJ130c validated for use in FC.
Research Category
Inflammation & Immunology
Research Sub Category
Immunoglobulins & Immunology

Quality

Evaluated by flow cytometry using human PBMCs

Target description

50-70 kDa (Tetteroo, 1987; Tamm, 1996)

Physical form

Protein A purified
PurifiedMouseConjugate in buffer containing Tris-HCl Other

Storage and Stability

Maintained refrigerated at 2-8°C from date of receipt. Protected from light in undiluted aliquots for 6 months from date of receipt.

Analysis Note

Control
PBMCs

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

MILLI-MARK is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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