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CBL56

Sigma-Aldrich

Anti-Adrenocorticotropic Hormone Antibody, CT, clone 56

clone 56, Chemicon®, from mouse

Synonym(s):

ACTH

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

100

antibody form

affinity purified immunoglobulin

antibody product type

primary antibodies

clone

56, monoclonal

species reactivity

rat, human

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
immunohistochemistry: suitable (paraffin)

isotype

IgG1

NCBI accession no.

NM_000939.2
NM_001035256.1

UniProt accession no.

P01189

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... MC2R(4158)
rat ... Mc2R(282839)

Specificity

This antibody is specific for CLIP (17-39 ACTH) and no cross reaction with Synacthen (1-24 ACTH).

KNOWN SPECIES CROSS REACTIVITY: Positive with rat C-terminal ACTH

Immunogen

ACTH-BSA Conjugate
Epitope: C-terminus

Application

Anti-Adrenocorticotropic Hormone Antibody, C-terminus, clone 56 is an antibody against Adrenocorticotropic Hormone for use in ELISA, IH(P).
Research Category
Neuroscience
Research Sub Category
CNS Control of Metabolism

Hormones & Receptors
This antibody reacts with solid phase ACTH either captured as part of a sandwich or adsorbed as a solid phase antigen and as such is suitable for serum assay development

The antibody is also reactive on formalin fixed paraffin wax embedded tissue and is therefore suitable for immunocytochemistry

Optimal working dilutions must be determined by the end user.

Physical form

Format: Purified
The monoclonal is presented as 1mg in 1ml phosphate buffered saline containing 0.1% sodium azide. We recommend that each laboratory determine an optimum working titre for use in its particular application.

Storage and Stability

For use within 1 month of purchase store at +4°C, for long term storage aliquot antibody into small volumes and store at -20°C.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

10 - Combustible liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Andrea Polito et al.
PloS one, 6(11), e25905-e25905 (2011-11-11)
The mechanisms of septic shock-associated adrenal insufficiency remain unclear. This study aimed at investigating the synthesis of corticotropin-releasing hormone (CRH) and vasopressin (AVP) by parvocellular neurons and the antehypophyseal expression of ACTH in human septic shock and in an experimental

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