CBL154

Anti-H-CAM Antibody, clone F10-44-2

clone F10-44-2, Chemicon^®, from mouse

• Synonym(s): CD44
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: F10-44-2, monoclonal
• Application: FACS, IHC (p), IP
• Citations: 6

Properties

• biological source: mouse
• Quality Segment: 100
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: F10-44-2, monoclonal
• species reactivity: human
• manufacturer/tradename: Chemicon^®
• technique(s): flow cytometry: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, immunoprecipitation (IP): suitable
• isotype: IgG2a
• NCBI accession no.: NM_000610.3, NM_001001389.1, NM_001001390.1, NM_001001391.1, NM_001001392.1
• UniProt accession no.: P16070
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... CD44(960)

Description

Application

Identification of CD44 positive cells by flow cytometry and immunocytochemistry, in particular with formalin fixed paraffin wax embedded sections of lymphoid and epithelial tissues (high temperature antigen retrieval in 10mM citrate buffer, pH6.0, is recommended).

Studies involving T, B cell, monocyte, granulocyte T and B cell binding to HEV during lymphocyte circulation and movement of leucocytes to inflammatory sites.

Studies on HCAM (homing receptor) function.

Immunoprecipitation.

Optimal working dilutions must be determined by the end user.

This Anti-H-CAM Antibody, clone F10-44-2 is validated for use in FC, IP, IH, IH(P) for the detection of H-CAM.

Physical form

Format: Purified

Analysis Note

Control
POSITIVE CONTROL: Tonsil; stains all T-cells in the paracortex

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 2