APT420
CaspaTag Pan-Caspase In Situ Assay Kit, Fluorescein
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product line
Chemicon®
species reactivity
mammals
technique(s)
activity assay: suitable
flow cytometry: suitable
Quality Level
1 of 4
This Item | APT400 | APT428 | APT409 |
|---|---|---|---|
| species reactivity mammals | species reactivity - | species reactivity - | species reactivity - |
| Quality Level 100 | Quality Level 100 | Quality Level 100 | Quality Level 100 |
| technique(s) activity assay: suitable, flow cytometry: suitable | technique(s) activity assay: suitable, flow cytometry: suitable | technique(s) activity assay: suitable, flow cytometry: suitable | technique(s) activity assay: suitable, flow cytometry: suitable |
| product line Chemicon® | product line - | product line - | product line - |
Application
The kit is for research use only. Not for use in diagnostic or therapeutic pr
General description
Caspases have been identified in organisms ranging from C. elegans to humans. The mammalian caspases play distinct roles in apoptosis and inflammation. In apoptosis, caspases are responsible for proteolytic cleavages that lead to cell disassembly (effector caspases), and are involved in upstream regulatory events (initiator caspases). An active caspase consists of two large and two small subunits that form two heterodimers, which associate in a tetramer2-4. In common with other proteases, caspases are synthesized as precursors that undergo proteolytic maturation, either autocatalytically or in a cascade by enzymes with similar specificity5.
Caspase enzymes specifically recognize a 4 or 5 amino acid sequence on the target substrate, which necessarily includes an aspartic acid residue. This residue is the target for cleavage, which occurs at the carbonyl end of the aspartic acid residue6. Caspases can be detected via immunoprecipitation, immunoblotting techniques using caspase specific antibodies, or by employing fluorochrome substrates, which become fluorescent upon cleavage by the caspase.
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signalword
Danger
Hazard Classifications
Acute Tox. 3 Inhalation - Acute Tox. 4 Dermal - Acute Tox. 4 Oral - Carc. 1B - Eye Irrit. 2 - Muta. 2 - Skin Irrit. 2 - Skin Sens. 1 - STOT SE 2 - STOT SE 3
target_organs
Eyes,Central nervous system, Respiratory system
Storage Class
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
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"Cells maintain a healthy state by delicately balancing multiple processes and signaling pathways. Only by studying all of these facets of cell health can we accurately characterize the state of a cell under specified conditions. Diverse inputs, such as disease states, nutrient availability, cell-cell contacts, extracellular matrix, tissue microenvironment, oxygen content, developmental cues, or various forms of cellular stress can shift the balance of cell health pathways. Therefore, a multipronged approach using complementary analysis platforms and technologies is necessary for a complete picture of cell health. In addition to using cell health assessment for elucidating disease mechanisms and therapeutic discovery, monitoring key indicators of cell health and performance (such as the apoptotic fraction, viability, cell cycle, cell counts, and transfection efficiency) helps establish uniform standards of cellular performance across long-term research studies. Knowing the performance profile of your cells prior to running your bioassay can mean the difference between valid assay results and wasted reagents, lost time and discarded data."
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