AP120A
Goat Anti-Human IgA Antibody, IgG, and IgM, Alkaline Phosphatase
Chemicon®, from goat
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.46
Recommended Products
biological source
goat
Quality Level
conjugate
alkaline phosphatase conjugate
antibody form
F(ab′)2 fragment of affinity isolated antibody
antibody product type
secondary antibodies
clone
polyclonal
species reactivity
human
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable
western blot: suitable
isotype
IgG
shipped in
wet ice
target post-translational modification
unmodified
General description
IgG is a monomeric immunoglobulin, built of two heavy chains and two light chains. Each molecule has two antigen binding sites. This is the most abundant immunoglobulin and is approximately equally distributed in blood and in tissue liquids, constituting 75% of serum immunoglobulins in humans. This is the only isotype that can pass through the human placenta, thereby providing protection to the fetus in its first weeks of life before its own immune system has developed. It can bind to many kinds of pathogens, for example viruses, bacteria, and fungi, and protects the body against them by complement activation (classic pathway), opsonization for phagocytosis and neutralisation of their toxins. There are 4 subclasses: IgG1 (66%), IgG2 (23%), IgG3 (7%) and IgG4 (4%).
IgM normally constitutes about 10% of serum immunoglobulins. IgM antibody is prominent in early immune responses to most antigens and predominates in certain antibody responses such as ′natural′ blood group antibodies. IgM (with IgD) is the major immunoglobulin expressed on the surface of B cells.
Monomeric IgA constitutes 5-15 % of the serum immunoglobulins whereas dimeric IgA is localized to mucosa surfaces such as saliva, gastrointestinal secretion, bronchial fluids and milk. Mucosal IgA plays a major role in host defence by neutralising infectious agents at mucosal surfaces. The production is usually local and antigen specific IgA producing B-cells can be found in regions under the lamina propria where they mature into dimeric IgA producing plasma cells. IgA deficiency is the most common immunodeficiency that may affect both serum and mucosal produced IgA.
IgM normally constitutes about 10% of serum immunoglobulins. IgM antibody is prominent in early immune responses to most antigens and predominates in certain antibody responses such as ′natural′ blood group antibodies. IgM (with IgD) is the major immunoglobulin expressed on the surface of B cells.
Monomeric IgA constitutes 5-15 % of the serum immunoglobulins whereas dimeric IgA is localized to mucosa surfaces such as saliva, gastrointestinal secretion, bronchial fluids and milk. Mucosal IgA plays a major role in host defence by neutralising infectious agents at mucosal surfaces. The production is usually local and antigen specific IgA producing B-cells can be found in regions under the lamina propria where they mature into dimeric IgA producing plasma cells. IgA deficiency is the most common immunodeficiency that may affect both serum and mucosal produced IgA.
Specificity
Specific for human IgG, IgM and IgA.
application
Goat anti-Human IgA Antibody, IgG & IgM, Alkaline Phosphatase detects level of Human IgA & has been published & validated for use in ELISA & WB.
Research Category
Secondary & Control Antibodies
Secondary & Control Antibodies
Research Sub Category
Whole Immunoglobulin Secondary Antibodies
Whole Immunoglobulin Secondary Antibodies
Western Blot:
1:5,000-1:50,000 dilution can be used.
Optimal working dilutions must be determined by the end user.
1:5,000-1:50,000 dilution can be used.
Optimal working dilutions must be determined by the end user.
Quality
Routinely evaluated by Enzyme-linked Immunosorbent Assay.
ELISA:
1:5,000-1:50,000 diltution can be used.
ELISA:
1:5,000-1:50,000 diltution can be used.
Physical form
ImmunoAffinity Purified
Purified goat secondary antibody IgG in lyophilized buffer containing 0.01 M Tris-HCL, 0.25 M NaCl, pH 8.0 with 15 mg/mL BSA and 0.05% sodium azide.
Storage and Stability
Store lyophilized material at 2-8°C for up to 1 year after date of receipt.
After reconstitution this product is stable for several weeks at 2-8°C as an undiluted liquid. DO NOT FREEZE. For extended storage after reconstitution, add an equal volume of glycerol (ACS grade or better) to make a final concentration of 50% glycerol and store at 2-8°C for up to 12 months.
Please note that the concentration of protein and buffer salts will decrease to one-half of the original after adding glycerol.
After reconstitution this product is stable for several weeks at 2-8°C as an undiluted liquid. DO NOT FREEZE. For extended storage after reconstitution, add an equal volume of glycerol (ACS grade or better) to make a final concentration of 50% glycerol and store at 2-8°C for up to 12 months.
Please note that the concentration of protein and buffer salts will decrease to one-half of the original after adding glycerol.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Warning
hcodes
Hazard Classifications
Acute Tox. 4 Dermal - Aquatic Chronic 3
Storage Class
11 - Combustible Solids
wgk_germany
WGK 3
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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