ABF133
Anti-PD-L1 Antibody/CD274
1 mg/mL, from rabbit
Synonym(s):
Programmed cell death 1 ligand 1, PD-L1, PDCD1 ligand 1, Programmed death ligand 1, B7 homolog 1, B7-H1, CD274
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
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biological source
rabbit
Quality Level
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
human
species reactivity (predicted by homology)
rhesus macaque (based on 100% sequence homology)
concentration
1 mg/mL
technique(s)
flow cytometry: suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... CD274(29126)
General description
Programmed cell death 1 ligand 1 (PD-L1/CD274) is a B7 related protein belonging to the BTN/MOG family. PDL-1/CD274 is essential for T-cell proliferation and production of IL10 and IFNG. PD-L1/CD274 reduces the secretion of IL-2 and IL-10 from memory T cells by inhibiting cell-mediated immune responses. PD-L1/CD274 may be involved in reducing allogenic CD4+ memory T-cell responses to endothelial cells. PD-L1/CD274 is highly expressed in heart, skeletal muscle, placenta, and lung and weakly expressed in thymus, spleen, kidney, and liver.
Specificity
This antibody should recognize isoforms 1 and 2 based upon immunogen design.
Immunogen
Epitope: This antibody recognizes the Ig-like C2-type domain in the extracellular domain of PD-L1/CD274.
KLH-conjugated linear peptide corresponding to the extracellular domain of human PD-L1/CD274.
Application
Anti-PD-L1 Antibody/CD274 is an antibody against PD-L1/CD274 for use in western blotting & flow cytometry.
Research Category
Inflammation & Immunology
Inflammation & Immunology
Research Sub Category
Immunological Signaling
Immunological Signaling
Western Blotting Analysis: 2 µg/mL from a representative lot detected PD-L1/CD274 in 10 µg of K562 cell lysate.
Flow Cytometry Analysis: 0.25 µg from a representative lot detected PD-L1/CD274 in 1X10E6 K562 cells.
Flow Cytometry Analysis: 0.25 µg from a representative lot detected PD-L1/CD274 in 1X10E6 K562 cells.
Quality
Evaluated by Western Blotting in Jurkat cell lysate.
Western Blotting Analysis: 2 µg/mL of this antibody detected PD-L1/CD274 in 10 µg of Jurkat cell lysate.
Western Blotting Analysis: 2 µg/mL of this antibody detected PD-L1/CD274 in 10 µg of Jurkat cell lysate.
Target description
~55 kDa and ~33 kDa observed. Uniprot describes three isoforms at 33 kDa, 20 kDa and 21 kDa This antibody contains 4 known glyscosylation sytes. An unglycosylated and glycosylated/membrane form may be observed at 34 kDa and 55 kDa, respectively.
Physical form
Affinity purified
Purified rabbit Polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Description
Pricing
Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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