ABE75

Anti-FOXP3 Antibody

serum, from rabbit

• Synonym(s): Forkhead box protein P3, Scurfin
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: rabbit
• Quality Level: 100
• antibody form: serum
• antibody product type: primary antibodies
• clone: polyclonal
• species reactivity: human, rat, mouse
• technique(s): immunocytochemistry: suitable; western blot: suitable
• isotype: IgG
• NCBI accession no.: NP_054728
• UniProt accession no.: Q9BZS1
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... FOXP3(50943) ; mouse ... Foxp3(20371) ; rat ... Foxp3(317382)

Description

General description

FOXP3 (forkhead box P3) is a member of the FOX protein family and is thought to function as the master regulator in the development and function of regulatory T cells. As a member of the forkhead/winged-helix family of transcriptional regulators FOXP3 is believed to regulate expression through DNA binding. Mutations and deletions in the sequence encoding for FOXP3 expression lead to an inability to express FOXP3, causing an inability to regulate T cell development and leading to fatal autoimmune diseases such as IPEX and XLAAD. Conversely, other studies have shown high and wide expression of FOXP3 in several cancer cell lines and tumors, indicating that FOXP3 is involved in preventing immunity against malignant tumors.

Immunogen

Linear peptide corresponding to human FOXP3.

Application

Immunocytochemistry Analysis: 1:500 dilution from a representative lot detected FOXP3 in A431 cells.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Transcription Factors

Use Anti-FOXP3 Antibody (Rabbit polyclonal antibody) validated in WB, ICC to detect FOXP3 also known as forkhead box P3.

Quality

Evaluated by Western Blot in HEK293 cell lysate.

Western Blot Analysis: 1:1,000 dilution of this antibody detected FOXP3 on 10 µg of HEK293 cell lysate.

Target description

~50 kDa observed

Physical form

Rabbit polyclonal serum containing 0.5% sodium azide.

Unpurified

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Analysis Note

Control
HEK293 cell lysate

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 1