Skip to Content
MilliporeSigma
All Photos(2)

Documents

ABE547

Sigma-Aldrich

Anti-ALKBH5 Antibody

from rabbit, purified by affinity chromatography

Synonym(s):

RNA demethylase ALKBH5, Alkylated DNA repair protein alkB homolog 5, Alpha-ketoglutarate-dependent dioxygenase alkB homolog 5

Sign Into View Organizational & Contract Pricing
All Photos(2)

About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

100

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

human

species reactivity (predicted by homology)

bovine (based on 100% sequence homology), canine (based on 100% sequence homology), rhesus macaque (based on 100% sequence homology), horse (based on 100% sequence homology)

technique(s)

immunoprecipitation (IP): suitable
western blot: suitable

NCBI accession no.

NP_060228.3

UniProt accession no.

Q6P6C2

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... ALKBH5(54890)

General description

RNA demethylase ALKBH5 is also known as Alkylated DNA repair protein alkB homolog 5 and Alpha-ketoglutarate-dependent dioxygenase alkB homolog 5. ALKBH5 is a dioxygenase that demethylates RNA, specifically N(6)-methyladenosine (m6A) RNA, which affects mRNA processing and export and is needed for spermatogenesis. ALKBH5 has the highest expression in lung, testis, pancreas, spleen and ovary, but is widely expressed across other tissues.

Immunogen

KLH-conjugated linear peptide corresponding to a region near the C-terminus of human ALKBH5.

Application

Detect ALKBH5 using this rabbit polyclonal antibody, Anti-ALKBH5 Antibody validated for use in western blotting & IP.
Immunoprecipitation Analysis: 10 µg from a representative lot detected ALKBH5 from 500 µg of MCF-7 cell lysate.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
RNA Metabolism & Binding Proteins

Quality

Evaluated by Western Blotting in MCF-7 cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected ALKBH5 in 10 µg of MCF-7 cell lysate.

Target description

~52 kDa observed. Uncharacterized band(s) may be observed in some cell lysates.

Physical form

Affinity purified
Purified rabbit polyclonal serum in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Not finding the right product?  

Try our Product Selector Tool.

recommended

Product No.
Description
Pricing

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Ye Yuan et al.
Cell death & disease, 12(1), 60-60 (2021-01-13)
ALKBH5 is the main enzyme for m6A-based demethylation of RNAs and it has been implicated in many biological and pathophysiological processes. Here, we aimed to explore the potential involvement of ALKBH5 in osteosarcoma and decipher the underlying cellular/molecular mechanisms. We
RNA demethylase ALKBH5 promotes colorectal cancer progression by posttranscriptional activation of RAB5A in an m6A-YTHDF2-dependent manner.
Shen, et al.
Clinical and Translational Medicine, 13, e1279-e1279 (2023)
Zhaofeng Li et al.
Cell death & disease, 12(6), 578-578 (2021-06-06)
N6-methyladenosine (m6A) modification is widespread in messenger RNAs and increasing evidence suggests the crucial roles of m6A in cell differentiation and tissue development. However, whether m6A modulates the osteogenic differentiation of mesenchymal stem cells (MSCs) has not been fully elucidated.
Wei Song et al.
Cell death discovery, 8(1), 25-25 (2022-01-16)
Long noncoding RNAs (lncRNAs) have crucial functions in the tumorigenesis and metastasis of cancers. N6-methyladenosine (m6A) modification of RNA is an important epigenetic regulatory mechanism in various malignancies. Nevertheless, the mechanism of m6A-modified lncRNA in diffuse large B cell lymphoma
Rodrigo A Carrasco et al.
Reproduction (Cambridge, England), 162(2), 171-179 (2021-06-16)
To elucidate the mechanism by which nerve growth factor (NGF) influences the LH secretory pathway in camelids, a series of experiments were done to determine the involvement of the hypothalamus (Experiment 1), the role of GnRH neurons (Experiment 2), and
View All Related Papers

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service