AB4332

Anti-acetyl-MVH (Lys405) Antibody

from rabbit, purified by affinity chromatography

• Synonym(s): Probable ATP-dependent RNA helicase DDX4, DEAD box protein 4, Mvh, Vasa homolog
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: rabbit
• Quality Level: 100
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• purified by: affinity chromatography
• species reactivity: pig, human, mouse
• species reactivity (predicted by homology): porcine (based on 100% sequence homology), rat (based on 100% sequence homology)
• technique(s): dot blot: suitable; immunocytochemistry: suitable; immunoprecipitation (IP): suitable; inhibition assay: suitable (peptide); western blot: suitable
• NCBI accession no.: NP_034159
• UniProt accession no.: Q61496
• shipped in: wet ice
• target post-translational modification: acetylation (Lys405)
• Gene Information: mouse ... Ddx4(13206)

Description

General description

MVH (also known as DDX4 or Vasa) is a member of the DEAD family of proteins and is a probable ATP-dependent RNA helicase. MVH is mostly localized in testicular germ cells in the cytoplasm of spermatocytes. MVH is a high-copy extragenic suppressor gene. MVH is shown to be significantly decreased in spermatozoa of men that are infertile and may have an important role in male fertility and in stem cell development.

Specificity

This antibody specifically recognizes wild type MVH acetylated at Lys405, but not the Lys405 mutant.

Immunogen

Epitope: Acetylated Lys405

KLH-conjugated linear peptide corresponding to mouse MVH acetylated at Lys405.

Application

Anti-acetyl-MVH (Lys405) Antibody is a Rabbit Polyclonal Antibody for detection of acetyl-MVH (Lys405) also known as Probable ATP-dependent RNA helicase DDX4, DEAD box protein & has been validated in DB, WB, ICC, PIA & IP.

Research Category
Stem Cell Research

Epigenetics & Nuclear Function

Research Sub Category
Cell Cycle, DNA Replication & Repair

Western Blot/Immunoprecipitation Analysis: A representative lot detected Flag-tagged acetyl-MVH (Lys405) in the presence of Myc-HAT/p46.

Immunocytochemistry Analysis: A representative lot from an independent laboratory detected acetyl-MVH (Lys405) in mouse germ cells (I. Nagamori, Department of Pharmacology, University of California, Irvine).

Quality

Evaluated by Dot Blot/Peptide Inhibition Assay in non-acetylated and acetylated MVH (Lys405).

Dot Blot/Peptide Inhibition Analysis: A 1:1,000 dilution of this antibody detected acetyl-MVH (Lys405) peptide.

Target description

76 kDa calculated

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Non-acetylated and acetylated MVH (Lys405)

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids