AB4251

Anti-CXC Chemokine Receptor 4 Antibody, NT, extracellular

Chemicon^®, from rabbit

• Synonym(s): CXCR4
• UNSPSC Code: 12352203
• eCl@ss: 32160702

Properties

• biological source: rabbit
• antibody form: affinity purified immunoglobulin
• antibody product type: primary antibodies
• clone: polyclonal
• purified by: affinity chromatography
• species reactivity: human
• manufacturer/tradename: Chemicon^®
• technique(s): immunohistochemistry: suitable (paraffin)
• NCBI accession no.: NM_001008540.1; NM_003467.2
• UniProt accession no.: P61073
• shipped in: dry ice
• target post-translational modification: unmodified
• Gene Information: human ... CXCR4(7852)

Description

General description

CXCR4 is a chemokine receptor involved in organ vascularization, neuronal cell migration, and patterning of the central nervous system during development. It binds stromal cell-derived factor 1 (SDF1) and mediates migration of resting leukocytes and is unique in homing hematopoietic progenitors to bone marrow. These properties suggest that CXCR4 is involved in tumor cell migration and local tumor invasion. It is also an HIV-1 fusion co-factor that allows HIV-1 invasion in diverse human cell types. CXCR4 has two isoforms that are produced by alternative splicing and has been reported to be expressed in cells of the immune and central nervous systems. It is highly expressed in brain and heart, and in white blood cells, vascular endothelial cells, and umbilical cord endothelial cells. In brain tumors, CXCR4 is expressed in regions of angiogenesis and degenerative, necrotic, and microcystic changes (Rempel et al., 2000). ESTs have been isolated from a wide variety of tissue libraries, particularly uterus, kidney, lung, tonsil, and brain.

Specificity

Recognizes human CXCR4. The specificity of this antibody has been confirmed by IHC staining of cardiac myocytes in human heart.

Immunogen

Epitope: N-terminal, extracellular

Synthetic peptide corresponding to a sequence within the N-terminal extracellular domain of human CXCR4. The immunogen has 93% homology with mouse and rat.

Application

Immunohistochemistry (formalin-fixed paraffin-embedded tissues)

Optimal working dilutions must be determined by the end user.

Research Category
Inflammation & Immunology

Research Sub Category
Cytokines & Cytokine Receptors

This Anti-CXC Chemokine Receptor 4 Antibody, N-terminal, extracellular is validated for use in IH(P) for the detection of CXC Chemokine Receptor 4.

Physical form

Peptide-affinity purified immunoglobulin. Liquid in phosphate buffered saline, pH 7.7, containing 0.01% sodium azide

Storage and Stability

Maintain at -70°C in undiluted aliquots for up to 12 months from date of receipt. May be stored at 2-8°C for short term use. Avoid repeated freeze/thaw cycles.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable