309825

Dihydrorhodamine 123

Cell-permeable fluorogenic probe that is useful for the detection of reactive oxygen species (ROS) such as peroxide and peroxynitrite.

• Synonym(s): Dihydrorhodamine 123
• Empirical Formula (Hill Notation): C₂₁H₁₈N₂O₃
• CAS Number: 109244-58-8
• Molecular Weight: 346.38
• MDL number: MFCD00285712
• UNSPSC Code: 12352116
• NACRES: NA.21

Properties

• Quality Level: 100
• assay: ≥95% (HPLC)
• form: solid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; protect from light
• color: pink-white
• solubility: DMSO: 5 mg/mL
• shipped in: ambient
• storage temp.: −20°C
• InChI: 1S/C21H18N2O3/c1-25-21(24)15-5-3-2-4-14(15)20-16-8-6-12(22)10-18(16)26-19-11-13(23)7-9-17(19)20/h2-11,20H,22-23H2,1H3
• InChI key: FNEZBBILNYNQGC-UHFFFAOYSA-N

Description

General description

Cell-permeable fluorogenic probe that is useful for detection of reactive oxygen species (ROS), such as peroxide and peroxynitrite.

Cell-permeable fluorogenic probe that is useful for the detection of reactive oxygen species (ROS) such as peroxide and peroxynitrite. Dihydrorhodamine is not fluorescent unless it is converted to rhodamine 123.

Biochem/physiol Actions

Cell permeable: yes

Primary Target
Detection of reactive oxygen species (ROS)

Product does not compete with ATP.

Reversible: no

Packaging

Packaged under inert gas

Warning

Toxicity: Standard Handling (A)

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Other Notes

Crow, J.P. 1997. Nitric Oxide1, 145.
Kim, Y.M., et al. 1996. Infect Immun. 64, 3074.
Williams, M.S., and Henkart, P.A. 1996. J. Immunol. 157, 2395.
van de Water, B., et al. 1995. Mol. Pharmacol. 48, 928.
Royall, J.A., and Ischiropoulos, H. 1993. Arch. Biochem. Biophys. 302, 348.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable