234405
Complement C5-Depleted Serum, Human
Synonym(s):
Complement Serum
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About This Item
UNSPSC Code:
12352202
NACRES:
NA.25
Recommended Products
form
liquid
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
avoid repeated freeze/thaw cycles
impurities
C5 antigen, none detected (Ouchterlony)
shipped in
dry ice
storage temp.
−70°C
General description
Human serum depleted of complement component C5 by affinity chromatography.
Human serum that has been depleted of complement component C5 by affinity chromatography. Intended for the detection and quantitation of hemolytically active C5 complement component. The classical and alternative pathways are intact except for the specifically depleted component. Recommended assay volume: ≤60 µl
Biochem/physiol Actions
Complement C5-depleted serum from humans produced by affinity chromatography aids the development of the complement cascade up to the desired convertase level. It eliminates the usual time-consuming, expensive, and stepwise formation of enzymatic complexes from purified products. Whole serum usage reduces the risk of protein inactivation during the process. It also promotes better physiological conditions. Complement C5-Depleted Serum is intended for the detection and quantitation of the hemolytically active C5 complement component. Creating complement convertases on erythrocytes enhances a better readout of the assay by spectrophotometric quantification of released hemoglobin.
Packaging
Please refer to vial label for lot-specific concentration.
Warning
Toxicity: Standard Handling (A)
Physical form
In 145 mM NaCl, 10 mM sodium phosphate, pH 7.3.
Preparation Note
Prepared from plasma of individuals that have been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.
Reconstitution
Following initial thaw, aliquot and freeze (-70°C).
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class
12 - Non Combustible Liquids
wgk_germany
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
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Customers Also Viewed
Marcin Okroj et al.
PloS one, 7(10), e47245-e47245 (2012-10-17)
C3 and C5 convertases are central stages of the complement cascade since they converge the different initiation pathways, augment complement activation by an amplification loop and lead to a common terminal pathway resulting in the formation of the membrane attack
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