208910
Anti-Calreticulin (405-417) Rabbit pAb
liquid, Calbiochem®
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
Recommended Products
biological source
rabbit
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
polyclonal
form
liquid
contains
≤0.1% sodium azide as preservative
species reactivity
monkey, human, mouse, canine, hamster, bovine, rat, porcine
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
avoid repeated freeze/thaw cycles
isotype
IgG
shipped in
wet ice
storage temp.
−20°C
target post-translational modification
unmodified
General description
Anti-Calreticulin (405-417), rabbit polyclonal, recognizes the ~63 kDa calreticulin in heat-shocked HeLa cells. It is validated for WB, ICC, IHC, and IP.
Protein A purified rabbit polyclonal antibody. Recognizes the ~63 kDa calreticulin protein.
Recognizes the ~63 kDa calreticulin protein in heat-shocked HeLa cells.
Immunogen
Human
a synthetic peptide [(C)EEEDVPGQAKDEL] corresponding to amino acids 405-417 of human calreticulin, conjugated to KLH
Application
Immunoblotting (1:500, mouse/rat; 1:1000, human, chemiluminescent)
Immunocytochemistry (see comments)
Immunoprecipitation (see comments)
Immunohistochemistry (see comments)
Immunocytochemistry (see comments)
Immunoprecipitation (see comments)
Immunohistochemistry (see comments)
Warning
Toxicity: Standard Handling (A)
Physical form
In PBS, 50% glycerol, pH 7.2.
Reconstitution
Following initial thaw, aliquot and freeze (-20°C).
Other Notes
Does not recognize calreticulin in Drosophila cells. This antibody has been reported to work for immunocytochemistry, immunohistochemistry, and immunoprecipitation. Variables associated with assay conditions will dictate the optimal working dilution.
Janicki, S., et al. 1997. J. Cell Biol. 139, 485.
Wada, I., et al. 1997. EMBO J. 16, 5420.
Nauseef, W.F., et al. 1995. J. Biol. Chem.270, 4741.
Jethmalani, S.M., et al. 1994. J. Biol. Chem. 269, 23603.
Smith, M.J., and Koch, G.L.E. 1989. EMBO J.8, 3581.
Wada, I., et al. 1997. EMBO J. 16, 5420.
Nauseef, W.F., et al. 1995. J. Biol. Chem.270, 4741.
Jethmalani, S.M., et al. 1994. J. Biol. Chem. 269, 23603.
Smith, M.J., and Koch, G.L.E. 1989. EMBO J.8, 3581.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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Storage Class
10 - Combustible liquids
wgk_germany
WGK 1
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Developmental cell, 21(6), 1171-1178 (2011-12-17)
Lysosomes move bidirectionally on microtubules, and this motility can be stimulated by overexpression of the small GTPase Arl8. By using affinity chromatography, we find that Arl8-GTP binds to the soluble protein SKIP (SifA and kinesin-interacting protein, aka PLEKHM2). SKIP was
Safa Lucken-Ardjomande Häsler et al.
The Journal of cell biology, 219(5) (2020-04-29)
In addition to the classical pathway of secretion, some transmembrane proteins reach the plasma membrane through alternative routes. Several proteins transit through endosomes and are exported in a Rab8-, Rab10-, and/or Rab11-dependent manner. GRAFs are membrane-binding proteins associated with tubules
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