196806

Bax Channel Blocker II, iMAC2 - CAS 335166-00-2 - Calbiochem

A MAC (Mitochondrial Apoptosis-induced Channel) inhibitor iMAC1 tructural analog that is reported to be less potent than iMAC1 in blocking Bid-induced cytochrome c release in cell-free mitochondria preparations.

• Synonym(s): Bax Channel Blocker II, iMAC2 - CAS 335166-00-2 - Calbiochem, (±)-3,6-Dibromo-9-(2-fluoro-3-piperazin-1-yl-propyl)-carbazole, 2HCl, MAC Inhibitor II
• Empirical Formula (Hill Notation): C₁₉H₂₀Br₂FN₃ · 2HCl
• CAS Number: 335166-00-2
• Molecular Weight: 542.11
• UNSPSC Code: 12352200

Properties

• Quality Level: 100
• assay: ≥95% (HPLC)
• form: solid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; desiccated (hygroscopic); protect from light
• color: off-white
• solubility: DMSO: 25 mg/mL
• shipped in: ambient
• storage temp.: 2-8°C

Description

General description

A MAC (Mitochondrial Apoptosis-induced Channel) inhibitor iMAC1 (Cat. No. 196805) structural analog that is reported to be less potent than iMAC1 in blocking Bid-induced cytochrome c release in cell-free mitochondria preparations (40% vs. 77% inhibition with 5 µM respective inhibitor), but more effective than iMAC1 in inhibiting STS- (Cat. No. 569396 & 569397) induced cytochrome c release in intact FL5.12 cells (Complete vs ~35% inhibition with 5 µM respective inhibitor), presumably due to an enhanced permeability. iMAC2 is also shown to be a superior blocker of MAC-mediated current flow in reconstituted mitochondrial outer membrane patches from apoptotic FL5.12 cells (IC₅₀ = 28 vs 300 nM in the case of iMAC2 and iMAC1, respectively) and protect FL5.12 from STS-induced apoptosis (IC₅₀ = 2.5 µM).

A MAC (Mitochondrial Apoptosis-induced Channel) inhibitor iMAC1 (Cat. No. 196805) structural analog that is reported to be less potent than iMAC1 in blocking Bid-induced cytochrome c release in cell-free mitochondria preparations (40% vs. 77% inhibition with 5 µM respective inhibitor), but more effective than iMAC1 in inhibiting STS- (Cat. No. 569396 & 569397) induced cytochrome c release in intact FL5.12 cells (Complete vs ~35% inhibition with 5 µM respective inhibitor), presumably due to an enhanced permeability. iMAC2 is also shown to be a superior blocker of MAC-mediated current flow in reconstituted mitochondrial outer membrane patches from apoptotic FL5.12 cells (IC₅₀ = 28 vs 300 nM in the case of iMAC2 and iMAC1, respectively) and protect FL5.12 from STS-induced apoptosis (IC₅₀ = 2.5 µM).

Packaging

Packaged under inert gas

Warning

Toxicity: Standard Handling (A)

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Other Notes

Peixoto, P.M., et al. 2009. Biochem. J.423, 381.
Bombrun, A., et al. 2003. J. Med. Chem.46, 4365.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class: 10-13 - German Storage Class 10 to 13