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Sigma-Aldrich

Anti-FAK Antibody, clone 4.47, Alexa Fluor™ 555 conjugate

clone 4.47, Upstate®, from mouse

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

100

conjugate

ALEXA FLUOR™ 555

antibody form

purified antibody

antibody product type

primary antibodies

clone

4.47, monoclonal

species reactivity

rat, mouse, human

manufacturer/tradename

Upstate®

technique(s)

flow cytometry: suitable
immunocytochemistry: suitable
western blot: suitable

isotype

IgG1

NCBI accession no.

NM_005607.3
NM_153831.2

UniProt accession no.

Q05397

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... PTK2(5747)

Related Categories

Specificity

p125FAK, does not cross react with Pyk-2

Immunogen

GST fusion protein corresponding to residues 1-423 of human FAK (focal adhesion kinase)

Application

Anti-FAK Antibody, clone 4.47, Alexa Fluor 555 conjugate detects level of FAK & has been published & validated for use in FC, WB & IC.
Research Category
Signaling
Research Sub Category
Cytoskeletal Signaling

Quality

Routinely evaluated by immunostaining in 3T3 cells

Target description

125kDa

Physical form

Protein G Purified

Storage and Stability

1 year at 4°C from date of shipment

Analysis Note

Control
Negative Control: Catalog # 16-240, Normal Mouse IgG, Alexa Fluor™ 488-conjugate.

Legal Information

ALEXA FLUOR is a trademark of Life Technologies
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Alexa Fluor™ is a registered trademark of Molecular Probes, Inc.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Advances in high content screening for drug discovery.
Giuliano, Kenneth A, et al.
Assay and Drug Development Technologies, 1, 565-577 (2003)
Jana Harizanova et al.
PloS one, 11(8), e0160591-e0160591 (2016-08-16)
Integrin adhesome proteins bind each other in alternative manners, forming within the cell diverse cell-matrix adhesion sites with distinct properties. An intriguing question is how such modular assembly of adhesion sites is achieved correctly solely by self-organization of their components.

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