biological source
human
Quality Level
mol wt
Mw 42 kDa
manufacturer/tradename
Upstate®
technique(s)
activity assay: suitable (kinase)
NCBI accession no.
UniProt accession no.
General description
Expressed by baculovirus in Sf21 insect cell.
N-terminal 6His-tagged recombinant human Tie2 residues 771-end.
Tyrosine 1108 has been identified as an autophosphorylation site in human Tie2. The Y1106F substitution in murine Tie2 (analogous to Y1108F in human Tie2) has been shown to block cell migration by disrupting binding of Tie2 to the docking protein Dok-R (Jones N., et al., Mol Cell Biol.(2003);23:2658-2668 and Murray B.W., et al., Biochemistry.(2001);40:10243-10253).
Application
Active, N-terminal 6His-tagged recombinant human Tie2 residues 771-end. For use in Kinase Assays.
Research Category
Inflammation & Immunology
Inflammation & Immunology
Biochem/physiol Actions
- Protein Target: Tie2
- Target Sub-Family:TK
Packaging
Also available in 250μg size-call for pricing and availability and reference catalog number 14-766M when ordering the 250μg size.
Quality
routinely evaluated by phosphorylation of Poly (Glu4-Tyr) (4:1)
Physical form
50mM Tris/HCl pH8.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij 35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
Format: Ni2+/NTA-agarose
Storage and Stability
Store -70°C. For maximum recovery of product, centrifuge original vial prior to removing the cap.
Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into pre-chilled microcentrifuge tubes and immediately s
Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into pre-chilled microcentrifuge tubes and immediately s
Other Notes
For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial
signalword
Warning
hcodes
Hazard Classifications
Skin Sens. 1
Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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