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ReadyTube® Fraser Broth

For enrichment of samples for Listeria sp. and Listeria monocytogene, pkg of (10 mL in 17 mL tube with blue cap (20 or 100 tubes per box))

Synonym(s):

Fraser Broth, Full Fraser Broth

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About This Item

UNSPSC Code:
41106225
NACRES:
NA.74

agency

ISO 11290

form

liquid (ready-to-use)

feature

ready-to-use

manufacturer/tradename

ReadyTube®

packaging

pkg of (10 mL in 17 mL tube with blue cap (20 or 100 tubes per box))

pH

7.2 ( in H2O)

application(s)

environmental
food and beverages
microbiology

storage temp.

2-8°C

suitability

Listeria spp.

Related Categories

General description

ReadyTube® is ready to use media to provide the highest level of quality and testing confidence for improving accuracy and significantly reducing the risk of false results. ReadyTube® Fraser broth is a selective enrichment medium designed to help the recovery of Listeria sp.

Application

ReadyTube® Fraser broth is used for the primary and secondary selective enrichment of Listeria sp. from food, animal feed as well as from environmental samples and other materials.

Analysis Note

This medium contains peptone, casein, yeast extract, and meat extract, which provide essential nutrients like carbon and nitrogenous compounds, including vitamins, amino acids, and trace ingredients. Phosphates buffer the medium while sodium chloride maintains osmotic equilibrium. Nalidixic acid and acriflavine inhibit the growth of Gram-negative and Gram-positive organisms respectively, except Listeria sp. Listeria sp. hydrolyze esculin to glucose and esculetin. The latter combines with ferric ions of ferric ammonium citrate, resulting in the formation of 6-7-Dihydroxycoumarin, a black-brown complex. Ferric ammonium citrate also enhances the growth of L. monocytogenes. The high salt tolerance (of sodium chloride) of Listeria is used as means to inhibit the growth of Enterococci. Lithium chloride is also used to inhibit Enterococci, which also possess the ability to hydrolyze esculin.

Legal Information

READYTUBE is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 2

Certificates of Analysis (COA)

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Articles

Detection of Listeria monocytogenes in Food and Environmental Samples

Detection of Listeria monocytogenes in food and environmental samples according to ISO 11290-1, FDA-BAM and USDA-FSIS

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